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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NSMCE2 Recombinant antibody (85188-4-RR)

    WB analysis using 85188-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85188-4-RR (NSMCE2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using NSMCE2 Recombinant monoclonal antibody (85188-4-RR)

    IHC staining of human testis using 85188-4-RR

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 85188-4-RR (NSMCE2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using NSMCE2 Recombinant antibody (85188-4-RR)

    IF Staining of U2OS using 85188-4-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NSMCE2 antibody (85188-4-RR, Clone: 242862D8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85188-4-RR

    Affinity and Kinetic Characterization of 85188-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85188-4-RR against Human NSMCE2 were performed. The affinity constant is below 1 pM.

NSMCE2 Recombinant monoclonal antibody

NSMCE2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85188-4-RR
Clone No.242862D8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

242862D8, C8orf36, E3 SUMO protein ligase NSE2, E3 SUMO-protein ligase NSE2, E3 SUMO-protein transferase NSE2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, A549 cells, U2OS cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85188-4-RR targets NSMCE2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag4545

Product name: Recombinant human NSMCE2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-247 aa of BC032797

Sequence: MPGRSSSNSGSTGFISFSGVESALSSLKNFQACINSGMDTASSVALDLVESQTEVSSEYSMDKAMVEFATLDRQLNHYVKAVQSTINHVKEERPEKIPDLKLLVEKKFLALQSKNSDADFQNNEKFVQFKQQLKELKKQCGLQADREADGTEGVDEDIIVTQSQTNFTCPITKEEMKKPVKNKVCGHTYEEDAIVRMIESRQKRKKKAYCPQIGCSHTDIRKSDLIQDEALRRAIENHNKKRHRHSE

相同性解析による交差性が予測される生物種
Full Name non-SMC element 2, MMS21 homolog (S. cerevisiae)
Calculated molecular weight 247 aa, 28 kDa
Observed molecular weight28-32 kDa
GenBank accession numberBC032797
Gene Symbol NSMCE2
Gene ID (NCBI) 286053
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ96MF7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NSMCE2 (E3 SUMO-protein ligase NSE2) has a C-terminal Siz/PIAS-RING (SP-RING) domain with E3 SUMO ligase activity (PMID: 15601841). NSMCE2, an essential SUMO ligase of the SMC5/6 complex suppresses cancer and aging in mice (PMID: 26443207).

Protocols

Product Specific Protocols
IF protocol for NSMCE2 antibody 85188-4-RRDownload protocol
IHC protocol for NSMCE2 antibody 85188-4-RRDownload protocol
WB protocol for NSMCE2 antibody 85188-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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