Validation Data Gallery
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Tested Applications
| Positive WB detected in | human SCFV Lv-Hv Transfected HEK-293T cells |
| Positive IHC detected in | Transfected HEK-293T cells Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:40000-1:160000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85170-1-RR targets GGGGS Linker in WB, IHC, ELISA applications and shows reactivity with recombinant samples.
| Tested Reactivity | recombinant |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | GGGGS Linker |
| Gene Symbol | |
| Gene ID (NCBI) | |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | GGGGSLINKER |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GGGGS is one of the best fold-breaking linkers to expose fusion protein partners. This antibody detects the protein that contains GGGGS linker.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GGGGS Linker antibody 85170-1-RR | Download protocol |
| WB protocol for GGGGS Linker antibody 85170-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |