Validation Data Gallery
Tested Applications
| Positive WB detected in | PC-3 cells, HeLa cells, MCF-7 cells, HT-29 cells, HT-1080 cells |
| Positive IF/ICC detected in | HT-29 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85139-9-RR targets EPHB4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg6504 Product name: Recombinant Human EPHB4 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 16-539 aa of NM_004444.4 Sequence: LEETLLNTKLETADLKWVTFPQVDGQWEELSGLDEEQHSVRTYEVCDVQRAPGQAHWLRTGWVPRRGAVHVYATLRFTMLECLSLPRAGRSCKETFTVFYYESDADTATALTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNVKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCAQLTVNLTRFPETVPRELVVPVAGSCVVDAVPAPGPSPSLYCREDGQWAEQPVTGCSCAPGFEAAEGNTKCRACAQGTFKPLSGEGSCQPCPANSHSNTIGSAVCQCRVGYFRARTDPRGAPCTTPPSAPRSVVSRLNGSSLHLEWSAPLESGGREDLTYALRCRECRPGGSCAPCGGDLTFDPGPRDLVEPWVVVRGLRPDFTYTFEVTALNGVSSLATGPVPFEPVNVTTDREVPPAVSDIRVTRSSPSSLSLAWAVPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSENRAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQLDESEGWREQLA 相同性解析による交差性が予測される生物種 |
| Full Name | EPH receptor B4 |
| Calculated molecular weight | 108 kDa |
| Observed molecular weight | 110-120 kDa |
| GenBank accession number | NM_004444.4 |
| Gene Symbol | EPHB4 |
| Gene ID (NCBI) | 2050 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P54760 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EPHB4 (Ephrin type-B receptor 4) is a member of the Eph receptor tyrosine kinase family, the largest group of receptor tyrosine kinases. It binds ephrin-B ligands to regulate embryonic vascular development, lymphangiogenesis, and adult angiogenesis. Aberrant EPHB4 signaling promotes tumor progression, metastasis, and therapy resistance in various cancers, including breast, prostate, and colorectal malignancies. As a key regulator of tumor vasculature and the tumor microenvironment, EPHB4 represents an emerging therapeutic target for anti-cancer drug development and a potential prognostic biomarker.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EPHB4 antibody 85139-9-RR | Download protocol |
| WB protocol for EPHB4 antibody 85139-9-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |