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Validation Data Gallery

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  • Western Blot (WB) analysis of U-251 cells using SYTL5 Recombinant antibody (85108-5-RR)

    WB analysis of U-251 using 85108-5-RR

    U-251 cells were subjected to SDS PAGE followed by western blot with 85108-5-RR (SYTL5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SK-N-SH cells using SYTL5 Recombinant antibody (85108-5-RR)

    WB analysis of SK-N-SH using 85108-5-RR

    SK-N-SH cells were subjected to SDS PAGE followed by western blot with 85108-5-RR (SYTL5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using SYTL5 Recombinant monoclonal antibody (85108-5-RR)

    IHC staining of rat brain using 85108-5-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 85108-5-RR (SYTL5 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using SYTL5 Recombinant antibody (85108-5-RR)

    IF Staining of rat brain using 85108-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using SYTL5 antibody (85108-5-RR, Clone: 242838D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using SYTL5 Recombinant antibody (85108-5-RR)

    IF Staining of rat brain using 85108-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using SYTL5 antibody (85108-5-RR, Clone: 242838D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85108-5-RR

    Affinity and Kinetic Characterization of 85108-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85108-5-RR against Human SYTL5 were performed. The affinity constant is below 1 pM.

SYTL5 Recombinant monoclonal antibody

SYTL5 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 85108-5-RR
Clone No.242838D12

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

242838D12, SLP5, synaptotagmin like 5, Synaptotagmin like protein 5, Synaptotagmin-like protein 5

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSK-N-SH cells, U-251 cells
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85108-5-RR targets SYTL5 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag21698

Product name: Recombinant human SYTL5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 56-343 aa of BC131585

Sequence: TQQEASRVCVHCHRNLGLIFDRGDPCQACSLRVCRECRVAGPNGSWKCTVCDKIAQLRIITGEWFFEEKAKRFKQVNVLGTDVVRQSILRRSPGAEEVQSQEQTRQDAEKSDTSPVAGKKASHDGPKRKGFLLSKFRSATRGEIITPKTDTGRSYSLDLDGQHFRSLKSPPGSDRGSTGSSDLNDQEPGPRTPKSSRSNGVTPGTQSSPAPSTRTVTSVISREYGFENSMDLAAIEGTSQELTKSHRRNTSGTPSIAVSGTSLSSDQSRSELDLSESFTEDSEDTVSI

相同性解析による交差性が予測される生物種
Full Name synaptotagmin-like 5
Calculated molecular weight 730 aa, 81 kDa
Observed molecular weight82 kDa
GenBank accession numberBC131585
Gene Symbol SYTL5
Gene ID (NCBI) 94122
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ8TDW5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SYTL5, also known as SLP5, is a member of the synaptotagmin-like protein (Slp) family. Members of this family are characterized by the presence of an N-terminal Slp homology domain (SHD), which functions as a Rab27-binding domain, and C-terminal tandem C2 domains, putative Ca(2+)-binding motifs. SYTL5 promotes the development and progression of papillary thyroid carcinoma (PTC) by activating the NF-κB signaling pathway (PMID: 36378561).

Protocols

Product Specific Protocols
IF protocol for SYTL5 antibody 85108-5-RRDownload protocol
WB protocol for SYTL5 antibody 85108-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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