Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
85098-1-PBS targets ND4 as part of a matched antibody pair:
MP02094-1: 85098-1-PBS capture and 85098-2-PBS detection (validated in Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | NADH dehydrogenase, subunit 4 (complex I) |
| Calculated molecular weight | 52 kDa |
| Observed molecular weight | 43-52 kDa |
| GenBank accession number | YP_003024035 |
| Gene Symbol | ND4 |
| Gene ID (NCBI) | 4538 |
| RRID | AB_3744010 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P03905 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
NADH dehydrogenase 4 (ND4) is one of the core subunits of mitochondrial respiratory chain Complex I and is essential for cellular energy production. Complex I is the first step involved in cellular aerobic respiration (i.e., oxidative phosphorylation), responsible for catalyzing the transfer of electrons from NADH to coenzyme Q10 (ubiquinone), thereby initiating the entire electron transport chain.