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Validation Data Gallery

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  • Western Blot (WB) analysis of MDA-MB-231 cells using TEAD4 Recombinant antibody (84932-4-RR)

    WB analysis of MDA-MB-231 using 84932-4-RR

    MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 84932-4-RR (TEAD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BGC-823 cells using TEAD4 Recombinant antibody (84932-4-RR)

    WB analysis of BGC-823 using 84932-4-RR

    BGC-823 cells were subjected to SDS PAGE followed by western blot with 84932-4-RR (TEAD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TEAD4 Recombinant antibody (84932-4-RR)

    IHC staining of human colon cancer using 84932-4-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84932-4-RR (TEAD4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TEAD4 Recombinant antibody (84932-4-RR)

    IHC staining of human colon cancer using 84932-4-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84932-4-RR (TEAD4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TEAD4 Recombinant antibody (84932-4-RR)

    IF Staining of HeLa using 84932-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TEAD4 antibody (84932-4-RR, Clone: 242496B2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84932-4-RR

    Affinity and Kinetic Characterization of 84932-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84932-4-RR against Human TEAD4 were performed. The affinity constant is 0.205 nM.

TEAD4 Recombinant monoclonal antibody

TEAD4 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84932-4-RR
Clone No.242496B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

242496B2, EFTR 2, hRTEF 1B, RTEF1, TCF13L1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMDA-MB-231 cells, BGC-823 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84932-4-RR targets TEAD4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag3059

Product name: Recombinant human TEAD4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 135-434 aa of BC015497

Sequence: SAQIISATAFHSSMALARGPGRPAVSGFWQGALPGQAGTSHDVKPFSQQTYAVQPPLPLPGFESPAGPAPSPSAPPAPPWQGRSVASSKLWMLEFSAFLEQQQDPDTYNKHLFVHIGQSSPSYSDPYLEAVDIRQIYDKFPEKKGGLKDLFERGPSNAFFLVKFWADLNTNIEDEGSSFYGVSSQYESPENMIITCSTKVCSFGKQVVEKVETEYARYENGHYSYRIHRSPLCEYMINFIHKLKHLPEKYMMNSVLENFTILQVVTNRDTQETLLCIAYVFEVSASEHGAQHHIYRLVKE

相同性解析による交差性が予測される生物種
Full Name TEA domain family member 4
Calculated molecular weight 434 aa, 48 kDa
Observed molecular weight50-55 kDa
GenBank accession numberBC015497
Gene Symbol TEAD4
Gene ID (NCBI) 7004
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ15561
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TEAD4 belongs to a protein family that shares the TEA/ATTS domain. The transcriptional activity of TEAD4 required several regions of the proteins, a TBP-associated factor, and a limiting transcriptional intermediary factor. TEAD4 also performs an essential role in the Hippo signaling pathway. TEAD4 regulates gene expression of YAP1 and WWTR1/TAZ, thus mediating cell proliferation, migration, and epithelial-mesenchymal transition induction. The calculated molecular weight of TEAD4 is 48 kDa, but the molecular weight of TEAD4 is detected at about 50-55 kDa.

Protocols

Product Specific Protocols
IF protocol for TEAD4 antibody 84932-4-RRDownload protocol
IHC protocol for TEAD4 antibody 84932-4-RRDownload protocol
WB protocol for TEAD4 antibody 84932-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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