Validation Data Gallery
Tested Applications
| Positive WB detected in | NIH/3T3 cells, Neuro-2a cells, C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84794-4-RR targets gp130/IL6ST in WB, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg1631 Product name: Recombinant Mouse gp130/IL6ST protein (rFc Tag)(HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 23-617 aa of NM_010560.3 Sequence: QLLEPCGYIYPEFPVVQRGSNFTAICVLKEACLQHYYVNASYIVWKTNHAAVPREQVTVINRTTSSVTFTDVVLPSVQLTCNILSFGQIEQNVYGVTMLSGFPPDKPTNLTCIVNEGKNMLCQWDPGRETYLETNYTLKSEWATEKFPDCQSKHGTSCMVSYMPTYYVNIEVWVEAENALGKVSSESINFDPVDKVKPTPPYNLSVTNSEELSSILKLSWVSSGLGGLLDLKSDIQYRTKDASTWIQVPLEDTMSPRTSFTVQDLKPFTEYVFRIRSIKDSGKGYWSDWSEEASGTTYEDRPSRPPSFWYKTNPSHGQEYRSVRLIWKALPLSEANGKILDYEVILTQSKSVSQTYTVTGTELTVNLTNDRYVASLAARNKVGKSAAAVLTIPSPHVTAAYSVVNLKAFPKDNLLWVEWTPPPKPVSKYILEWCVLSENAPCVEDWQQEDATVNRTHLRGRLLESKCYQITVTPVFATGPGGSESLKAYLKQAAPARGPTVRTKKVGKNEAVLAWDQIPVDDQNGFIRNYSISYRTSVGKEMVVHVDSSHTEYTLSSLSSDTLYMVRMAAYTDEGGKDGPEFTFTTPKFAQGEIE 相同性解析による交差性が予測される生物種 |
| Full Name | interleukin 6 signal transducer |
| Calculated molecular weight | 102kDa, 917aa |
| Observed molecular weight | 140 kDa |
| GenBank accession number | NM_010560.3 |
| Gene Symbol | Il6st |
| Gene ID (NCBI) | 16195 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q00560 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GP130, also known as IL6ST, is a transmembrane protein that serves as a shared signal-transducing subunit for a variety of cytokines(PMID: 39414364), including interleukin-6 (IL-6), IL-11, IL-27, leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), oncostatin M (OSM), cardiotrophin-1 (CT-1), cardiotrophin-like cytokine (CLC)/CLC factor 1 (CLCF-1), and neuropoietin (PMID: 24099136). This protein is highly conserved among vertebrates and plays a crucial role in mediating interactions between a wide range of immune and non-immune cells. GP130 was originally discovered in 1978 and was successfully cloned and expressed in 1990, leading to a deeper understanding of cytokine signaling pathways (PMID: 308233).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for gp130/IL6ST antibody 84794-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |