Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse spleen tissue |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84790-2-RR targets Siglec-E in WB, IHC, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg2733 Product name: Recombinant Mouse Siglec-12 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 19-350 aa of NM_031181.2 Sequence: QNPQEVFTLNVERKVVVQEGLCVLVPCNFSYLKKRLTDWTDSDPVHGFWYREGTDRRKDSIVATNNPIRKAVKETRNRFFLLGDPWRNDCSLNIREIRKKDAGLYFFRLERGKTKYNYMWDKMTLVVTALTNTPQILLPETLEAGHPSNLTCSVPWDCGWTAPPIFSWTGTSVSFLSTNTTGSSVLTITPQPQDHGTNLTCQVTLPGTNVSTRMTIRLNVSYAPKNLTVTIYQGADSVSTILKNGSSLPISEGQSLRLICSTDSYPPANLSWSWDNLTLCPSKLSKPGLLELFPVHLKHGGVYTCQAQHALGSQHISLSLSPQSSATLSEMM 相同性解析による交差性が予測される生物種 |
| Full Name | sialic acid binding Ig-like lectin E |
| Calculated molecular weight | 52 kDa |
| Observed molecular weight | 70-90 kDa |
| GenBank accession number | NM_031181.2 |
| Gene Symbol | Siglec-E |
| Gene ID (NCBI) | 83382 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q91Y57 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily that contain sialoside-binding N-terminal domains (PMID: PMID: 9498912; 22749793). Mouse Siglec‑E, also known as myeloid inhibitory siglec (MIS), is a mouse CD33-related siglec that contains three extracellular immunoglobulin-like domains, a transmembrane region and a cytoplasmic tail bearing two immunoreceptor tyrosine-based inhibitory motifs (ITIMs), which can recruit SHP-1 and SHP-2, two inhibitory regulators of immunoreceptor signal transduction (PMID: 11171044). Siglec-E is an inhibitory receptor and is expressed broadly on cells of innate immune cells including neutrophils, monocytes, macrophages, dendritic cells, and a subset of natural killer cells (PMID: 15048729; 31546700).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Siglec-E antibody 84790-2-RR | Download protocol |
| WB protocol for Siglec-E antibody 84790-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |