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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using C11orf68 Recombinant antibody (84725-5-RR)

    WB analysis of A431 using 84725-5-RR

    A431 cells were subjected to SDS PAGE followed by western blot with 84725-5-RR (C11orf68 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using C11orf68 Recombinant antibody (84725-5-RR)

    WB analysis of PC-3 using 84725-5-RR

    PC-3 cells were subjected to SDS PAGE followed by western blot with 84725-5-RR (C11orf68 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using C11orf68 Recombinant antibody (84725-5-RR)

    IHC staining of human stomach cancer using 84725-5-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84725-5-RR (C11orf68 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using C11orf68 Recombinant antibody (84725-5-RR)

    IF Staining of A431 using 84725-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using C11orf68 antibody (84725-5-RR, Clone: 242221C11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U2OS cells using C11orf68 Recombinant antibody (84725-5-RR)

    FC experiment of U2OS using 84725-5-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug C11orf68 Recombinant antibody (84725-5-RR, Clone:242221C11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 84725-5-RR

    Affinity and Kinetic Characterization of 84725-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84725-5-RR against Human C11orf68 were performed. The affinity constant is 11.8 nM.

C11orf68 Recombinant monoclonal antibody

C11orf68 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84725-5-RR
Clone No.242221C11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

242221C11, BLES03, P5326, Protein p5326, UPF0696 protein C11orf68

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inA431 cells, PC-3 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84725-5-RR targets C11orf68 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag36023

Product name: Recombinant human C11orf68 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-251 aa of BC010512

Sequence: MEPGEELEEEGSPGGREDGFTAEHLAAEAMAADMDPWLVFDARTTPATELDAWLAKYPPSQVTRYGDPGSPNSEPVGWIAVYGQGYSPNSGDVQGLQAAWEALQTSGRPITPGTLRQLAITHHVLSGKWLMHLAPGFKLDHAWAGIARAVVEGRLQVAKVSPRAKEGGRQVICVYTDDFTDRLGVLEADSAIRAAGIKCLLTYKPDVYTYLGIYRANRWHLCPTLYESRFQLGGSARGSRVLDRANNVELT

相同性解析による交差性が予測される生物種
Full Name chromosome 11 open reading frame 68
Observed molecular weight35 kDa
GenBank accession numberBC010512
Gene Symbol C11orf68
Gene ID (NCBI) 83638
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9H3H3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BLES03 (basophilic leukemia-expressed protein 03) is a human protein of unknown function. Recent studies have shown that BLES03 is a novel eukaryotic phosphothreonine cleavage enzyme involved in the biosynthesis of dehydrogenated amino acids.The crystal structure of BLES03 has been resolved by single-wavelength anomalous scattering (SAD) with a resolution of 2.5 Å.

Protocols

Product Specific Protocols
WB protocol for C11orf68 antibody 84725-5-RRDownload protocol
IHC protocol for C11orf68 antibody 84725-5-RRDownload protocol
IF protocol for C11orf68 antibody 84725-5-RRDownload protocol
FC protocol for C11orf68 antibody 84725-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols