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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using C11orf68 Recombinant antibody (84725-4-RR)

    WB analysis of A431 using 84725-4-RR

    WB result of C11orf68 antibody (84725-4-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C11orf68 transfected A431 cells.

  • Western Blot (WB) analysis of various lysates using C11orf68 Recombinant antibody (84725-4-RR)

    WB analysis using 84725-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84725-4-RR (C11orf68 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84725-4-RR

    Affinity and Kinetic Characterization of 84725-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84725-4-RR against Human C11orf68 were performed. The affinity constant is 22.8 nM.

  • Featured Product
  • KD/KO Validated

C11orf68 Recombinant monoclonal antibody

C11orf68 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 84725-4-RR
Clone No.242221F8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

242221F8, BLES03, P5326, Protein p5326, UPF0696 protein C11orf68

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inA431 cells, mouse liver tissue, rat pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84725-4-RR targets C11orf68 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag36023

Product name: Recombinant human C11orf68 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-251 aa of BC010512

Sequence: MEPGEELEEEGSPGGREDGFTAEHLAAEAMAADMDPWLVFDARTTPATELDAWLAKYPPSQVTRYGDPGSPNSEPVGWIAVYGQGYSPNSGDVQGLQAAWEALQTSGRPITPGTLRQLAITHHVLSGKWLMHLAPGFKLDHAWAGIARAVVEGRLQVAKVSPRAKEGGRQVICVYTDDFTDRLGVLEADSAIRAAGIKCLLTYKPDVYTYLGIYRANRWHLCPTLYESRFQLGGSARGSRVLDRANNVELT

相同性解析による交差性が予測される生物種
Full Name chromosome 11 open reading frame 68
Observed molecular weight35 kDa
GenBank accession numberBC010512
Gene Symbol C11orf68
Gene ID (NCBI) 83638
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9H3H3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BLES03 (basophilic leukemia-expressed protein 03) is a human protein of unknown function. Recent studies have shown that BLES03 is a novel eukaryotic phosphothreonine cleavage enzyme involved in the biosynthesis of dehydrogenated amino acids.The crystal structure of BLES03 has been resolved by single-wavelength anomalous scattering (SAD) with a resolution of 2.5 Å.

Protocols

Product Specific Protocols
WB protocol for C11orf68 antibody 84725-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols