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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C3orf37 Recombinant antibody (84660-4-RR)

    WB analysis using 84660-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84660-4-RR (C3orf37 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using C3orf37 Recombinant monoclonal antibody (84660-4-RR)

    IF Staining of MCF-7 using 84660-4-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using C3orf37 antibody (84660-4-RR, Clone: 242156E11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

C3orf37 Recombinant monoclonal antibody

C3orf37 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84660-4-RR
Clone No.242156E11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

HMCES, 242156E11, DC12, SRAPD1, UPF0361 protein C3orf37

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84660-4-RR targets C3orf37 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag36362

Product name: Recombinant human C3orf37 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 100-300 aa of BC009993

Sequence: DTVMEKRSFKVPLGKGRRCVVLADGFYEWQRCQGTNQRQPYFIYFPQIKTEKSGSIGAADSPENWEKVWDNWRLLTMAGIFDCWEPPEGGDVLYSYTIITVDSCKGLSDIHHRMPAILDGEEAVSKWLDFGEVSTQEALKLIHPTENITFHAVSSVVNNSRNNTPECLAPVDLVVKKELRASGSSQRMLQWLATKSPKKED

相同性解析による交差性が予測される生物種
Full Name chromosome 3 open reading frame 37
Observed molecular weight39 kDa
GenBank accession numberBC009993
Gene Symbol C3orf37
Gene ID (NCBI) 56941
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ96FZ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HMCES, also known as C3orf37, is a protein that is associated with the protection of abasic sites. The molecular mass of HMCES is 40 kDa and it recognizes AP sites in ssDNA at replication forks and chemically modifies the lesion to generate a DNA-protein crosslink (PMID: 30554877).

Protocols

Product Specific Protocols
IF protocol for C3orf37 antibody 84660-4-RRDownload protocol
WB protocol for C3orf37 antibody 84660-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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