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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C12orf51 Recombinant antibody (84641-1-RR)

    WB analysis using 84641-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84641-1-RR (C12orf51 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using C12orf51 Recombinant antibody (84641-1-RR)

    IHC staining of human colon cancer using 84641-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84641-1-RR (C12orf51 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using C12orf51 Recombinant antibody (84641-1-RR)

    IHC staining of human colon cancer using 84641-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84641-1-RR (C12orf51 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84641-1-RR

    Affinity and Kinetic Characterization of 84641-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84641-1-RR against Human C12orf51 were performed. The affinity constant is below 1 pM.

C12orf51 Recombinant monoclonal antibody

C12orf51 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 84641-1-RR
Clone No.241815A12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

HECTD4, 241815A12, HECT domain-containing protein 4, HECT-type E3 ubiquitin transferase HECTD4, KIAA0614

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA375 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84641-1-RR targets C12orf51 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag14614

Product name: Recombinant human C12orf51 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 3723-3996 aa of BC006270

Sequence: TSFRKTLVGEPLDPEQDLQEADILTYNYVKKFESINDETELEALCAEIASQHLATESPDSPNKPCCRFTYLTMTGEEVELCSRGRHILVAWENKDIYAAAIRSLRLRELQNVECVTAVRAGLGSIIPLQLLTMLSPLEMELRTCGLPYINLEFLKAHTMYQVGLMETDQHIEFFWGALEMFTQEELCKFIKFACNQERIPFTCPCKDGGPDTAHVPPYPMKIAPPDGTAGSPDSRYIRVETCMFMIKLPQYSSLEIMLEKLRCAIHYREDPLSG

相同性解析による交差性が予測される生物種
Full Name chromosome 12 open reading frame 51
Calculated molecular weight 3996 aa, 439 kDa
Observed molecular weight500 kDa
GenBank accession numberBC006270
Gene Symbol C12orf51
Gene ID (NCBI) 283450
RRIDAB_3672100
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y4D8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The HECT domain E3 ubiquitin protein ligase 4 (HECTD4), is also known as C12orf51. It may be involved in protein modification or ubiquitination (PMID: 26891264). In addition, genetic polymorphisms of C12orf51 are related to alcohol consumption and type 2 diabetes (T2D) (PMID: 38523829).

Protocols

Product Specific Protocols
WB protocol for C12orf51 antibody 84641-1-RRDownload protocol
IHC protocol for C12orf51 antibody 84641-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols