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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse liver tissue using INTS6 Recombinant antibody (84610-3-RR)

    IHC staining of mouse liver using 84610-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84610-3-RR (INTS6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using INTS6 Recombinant antibody (84610-3-RR)

    IHC staining of mouse liver using 84610-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84610-3-RR (INTS6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using INTS6 Recombinant antibody (84610-3-RR)

    IF Staining of HeLa using 84610-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using INTS6 antibody (84610-3-RR, Clone: 242099E6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U-251 cells using INTS6 Recombinant antibody (84610-3-RR)

    FC experiment of U-251 using 84610-3-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug INTS6 Recombinant antibody (84610-3-RR, Clone:242099E6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 84610-3-RR

    Affinity and Kinetic Characterization of 84610-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84610-3-RR against Human INTS6 were performed. The affinity constant is 1.71 nM.

INTS6 Recombinant monoclonal antibody

INTS6 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84610-3-RR
Clone No.242099E6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

DICE1, DDX26A, DDX26, DBI1, DBI 1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU-251 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84610-3-RR targets INTS6 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag36146

Product name: Recombinant human INTS6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 672-815 aa of BC039829

Sequence: VVNNHIGGKGPPAPTTQAQPDLIKPLPLHKISETTNDSIIHDVVENHVADQLSSDITPNAMDTEFSASSPASLLERPTNHMEALGHDHLGTNDLTVGGFLENHEEPRDKEQCAEENIPASSLNKGKKLMHCRSHEEVNTELKAQ

相同性解析による交差性が予測される生物種
Full Name integrator complex subunit 6
Calculated molecular weight 100 kDa
GenBank accession numberBC039829
Gene Symbol INTS6
Gene ID (NCBI) 26512
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9UL03
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

INTS6 is a component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing.

Protocols

Product Specific Protocols
IF protocol for INTS6 antibody 84610-3-RRDownload protocol
IHC protocol for INTS6 antibody 84610-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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