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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INTS6 Recombinant antibody (84610-1-RR)

    WB analysis using 84610-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84610-1-RR (INTS6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using INTS6 Recombinant antibody (84610-1-RR)

    IF Staining of HeLa using 84610-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using INTS6 antibody (84610-1-RR, Clone: 242099C7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using INTS6 Recombinant antibody (84610-1-RR)

    IF Staining of HeLa using 84610-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using INTS6 antibody (84610-1-RR, Clone: 242099C7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84610-1-RR

    Affinity and Kinetic Characterization of 84610-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84610-1-RR against Human INTS6 were performed. The affinity constant is 0.63 nM.

INTS6 Recombinant monoclonal antibody

INTS6 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84610-1-RR
Clone No.242099C7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

242099C7, DBI 1, DBI1, DDX26, DDX26A

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-3 cells, DU 145 cells, HuH-7 cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84610-1-RR targets INTS6 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag36146

Product name: Recombinant human INTS6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 672-815 aa of BC039829

Sequence: VVNNHIGGKGPPAPTTQAQPDLIKPLPLHKISETTNDSIIHDVVENHVADQLSSDITPNAMDTEFSASSPASLLERPTNHMEALGHDHLGTNDLTVGGFLENHEEPRDKEQCAEENIPASSLNKGKKLMHCRSHEEVNTELKAQ

相同性解析による交差性が予測される生物種
Full Name integrator complex subunit 6
Calculated molecular weight 100 kDa
Observed molecular weight130 kDa
GenBank accession numberBC039829
Gene Symbol INTS6
Gene ID (NCBI) 26512
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9UL03
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

INTS6 is a component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing.

Protocols

Product Specific Protocols
IF protocol for INTS6 antibody 84610-1-RRDownload protocol
WB protocol for INTS6 antibody 84610-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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