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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using VPS28 Recombinant monoclonal antibody (84472-4-RR)

    WB analysis of HeLa using 84472-4-RR

    WB result of VPS28 antibody (84472-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS28 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using VPS28 Recombinant antibody (84472-4-RR)

    WB analysis using 84472-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84472-4-RR (VPS28 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using VPS28 Recombinant antibody (84472-4-RR)

    IF Staining of A549 using 84472-4-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using VPS28 antibody (84472-4-RR, Clone: 241886D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using VPS28 Recombinant antibody (84472-4-RR)

    FC experiment of SH-SY5Y using 84472-4-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug VPS28 Recombinant antibody (84472-4-RR, Clone:241886D3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 84472-4-RR

    Affinity and Kinetic Characterization of 84472-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84472-4-RR against Human VPS28 were performed. The affinity constant is 1.72 nM.

  • Featured Product
  • KD/KO Validated

VPS28 Recombinant monoclonal antibody

VPS28 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84472-4-RR
Clone No.241886D3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

241886D3, ESCRT-I complex subunit VPS28, H-Vps28, Vacuolar protein sorting-associated protein 28 homolog

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa cells, A549 cells, Jurkat cells, THP-1 cells, U-251 cells
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84472-4-RR targets VPS28 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag7784

Product name: Recombinant human VPS28 protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 1-221 aa of BC006485

Sequence: MFHGIPATPGIGAPGNKPELYEEVKLYKNAREREKYDNMAELFAVVKTMQALEKAYIKDCVSPSEYTAACSRLLVQYKAAFRQVQGSEISSIDEFCRKFRLDCPLAMERIKEDRPITIKDDKGNLNRCIADVVSLFITVMDKLRLEIRAMDEIQPDLRELMETMHRMSHLPPDFEGRQTVSQWLQTLSGMSASDELDDSQVRQMLFDLESAYNAFNRFLHA

相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 28 homolog (S. cerevisiae)
Calculated molecular weight 25 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC006485
Gene Symbol VPS28
Gene ID (NCBI) 51160
RRIDAB_3671995
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9UK41
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VPS28 is a subunit of the ESCRT-I complex (endosomal sorting complex I required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. The ESCRT-I complex is composed of VPS23 (also known as TSG101), VPS28, VPS37, and MVB12A or MVB12B. VPS28 interacts with VPS23. Two alternative transcripts encoding different VPS28 isoforms have been described.

Protocols

Product Specific Protocols
FC protocol for VPS28 antibody 84472-4-RRDownload protocol
IF protocol for VPS28 antibody 84472-4-RRDownload protocol
WB protocol for VPS28 antibody 84472-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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