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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using 5'-Nucleotidase/CD73 Recombinant antibody (84434-4-RR)

    WB analysis of mouse brain using 84434-4-RR

    mouse brain tissue was subjected to SDS PAGE followed by western blot with 84434-4-RR (5'-Nucleotidase/CD73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of 4T1 cells using CD73 Recombinant antibody (84434-4-RR)

    IF Staining of 4T1 using 84434-4-RR

    Immunofluorescent analysis of (4% PFA) fixed 4T1 cells using 5'-Nucleotidase/CD73 antibody (84434-4-RR, Clone: 241541F5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84434-4-RR

    Affinity and Kinetic Characterization of 84434-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84434-4-RR against Mouse CD73 were performed. The affinity constant is below 1 pM.

CD73 Recombinant monoclonal antibody

CD73 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84434-4-RR
Clone No.241541F5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Ecto-5'-nucleotidase, EC:3.1.3.99, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse brain tissue
Positive IF/ICC detected in4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84434-4-RR targets CD73 in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

FusionProtein

相同性解析による交差性が予測される生物種
Full Name 5' nucleotidase, ecto
Calculated molecular weight64kDa
Observed molecular weight60 kDa
GenBank accession numberNM_011851.4
Gene Symbol Nt5e
Gene ID (NCBI) 23959
RRIDAB_3671960
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ61503
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).

Protocols

Product Specific Protocols
WB protocol for CD73 antibody 84434-4-RRDownload protocol
IF protocol for CD73 antibody 84434-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols