Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, SH-SY5Y cells, HepG2 cells, U2OS cells, PC-3 cells, MDA-MB-231 cells, NIH/3T3 cells |
| Positive IHC detected in | mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84366-5-RR targets EPN2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag35194 Product name: Recombinant human EPN2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 252-448 aa of BC093972 Sequence: LEESRRDTVKIPKKKEHGSLPQQTTLLDLMDALPSSGPAAQKAEPWGPSASTNQTNPWGGPAAPASTSDPWPSFGTKPAASIDPWGVPTGATAQSVPKNSDPWAASQQPASSAGKRASDAWGAVSTTKPVSVSGSFELFSNLNGTIKDDFSEFDNLRTSKKTAESVTSLPSQNNGTTSPDPFESQPLTVASSKPSSA 相同性解析による交差性が予測される生物種 |
| Full Name | epsin 2 |
| Calculated molecular weight | 584 aa, 62 kDa |
| Observed molecular weight | 62 kDa |
| GenBank accession number | BC093972 |
| Gene Symbol | EPN2 |
| Gene ID (NCBI) | 22905 |
| RRID | AB_3671902 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | O95208 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EPN2 (EPS 15 interacting protein 2) is a protein that interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. EPN2 is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for EPN2 antibody 84366-5-RR | Download protocol |
| WB protocol for EPN2 antibody 84366-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |