Validation Data Gallery
Tested Applications
| Positive WB detected in | rat brain tissue, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84290-6-RR targets RINT1 in WB, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag6145 Product name: Recombinant human RINT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 437-792 aa of BC068483 Sequence: RWLTVERKFALQKMDSMLSSEAAWVSQYKDITDVDEMKVPDCAETFMTLLLVITDRYKNLPTASRKLQFLELQKDLVDDFRIRLTQVMKEETRASLGFRYCAILNAVNYISTVLADWADNVFFLQLQQAALEVFAENNTLSKLQLGQLASMESSVFDDMINLLERLKHDMLTRQVDHVFREVKDAAKLYKKERWLSLPSQSEQAVMSLSSSACPLLLTLRDHLLQLEQQLCFSLFKIFWQMLVEKLDVYIYQEIILANHFNEGGAAQLQFDMTRNLFPLFSHYCKRPENYFKHIKEACIVLNLNVGSALLLKDVLQSASGQLPATAALNEVGIYKLAQQDVEILLNLRTNWPNTGK 相同性解析による交差性が予測される生物種 |
| Full Name | RAD50 interactor 1 |
| Calculated molecular weight | 91 kDa |
| Observed molecular weight | 87 kDa |
| GenBank accession number | BC068483 |
| Gene Symbol | RINT1 |
| Gene ID (NCBI) | 60561 |
| RRID | AB_3671835 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q6NUQ1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RINT-1 was first identified as a Rad50-interacting protein that participates in radiation-induced G2/M checkpoint control. It also involved in regulation of membrane traffic between the endoplasmic reticulum and the Golgi. It interacts with p130, an Rb family protein, and together they regulate the telomerase-independent telomere length. Otherwise, it interacts with Zeste white 10 with its N-terminal region throughout the cell cycle.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RINT1 antibody 84290-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |