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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LIMPII Recombinant antibody (84193-4-RR)

    WB analysis using 84193-4-RR (same clone as 84193-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84193-4-RR (LIMPII antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84193-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using LIMPII Recombinant antibody (84193-4-RR)

    WB analysis of HepG2 using 84193-4-RR (same clone as 84193-4-PBS)

    WB result of LIMPII antibody (84193-4-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-LIMPII transfected HepG2 cells. This data was developed using the same antibody clone with 84193-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01086-4

    Sandwich ELISA standard curve of MP01086-4

    Sandwich ELISA standard curve of MP01086-4, Human LIMPII Recombinant Matched Antibody Pair - PBS only. 84193-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag25908. 84193-3-PBS was HRP conjugated as the detection antibody. Range: 3.91-250 ng/mL.

  • Sample test of MP01086-4

    Sample test of MP01086-4

    The mean LIMPII concentration was determined to be 271.42 ng/mL in mouse liver tissue extract based on a 3.70 mg/mL extract load.

  • Affinity and Kinetic Characterization of 84193-4-PBS

    Affinity and Kinetic Characterization of 84193-4-PBS

    Biolayer interferometry (BLl) kinetic assays of 84193-4-PBS against Human LIMPII were performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of 84193-4-RR

    Affinity and Kinetic Characterization of 84193-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84193-4-RR against Human LIMPII were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

LIMPII Recombinant monoclonal antibody, PBS Only (Capture)

LIMPII Uni-rAb® Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84193-4-PBS
Clone No.241468F9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

SCARB2, 241468F9, AMRF, CD36, CD36 antigen like 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84193-4-PBS targets LIMPII as part of a matched antibody pair:

MP01086-4: 84193-4-PBS capture and 84193-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag25908

Product name: Recombinant human LIMPII protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 249-433 aa of BC021892

Sequence: NGTDGDSFHPLITKDEVLYVFPSDFCRSVYITFSDYESVQGLPAFRYKVPAEILANTSDNAGFCIPEGNCLGSGVLNVSICKNGAPIIMSFPHFYQADERFVSAIEGMHPNQEDHETFVDINPLTGIILKAAKRFQINIYVKKLDDFVETGDIRTMVFPVMYLNESVHIDKETASRLKSMINTTL

相同性解析による交差性が予測される生物種
Full Name scavenger receptor class B, member 2
Calculated molecular weight 478 aa, 54 kDa
Observed molecular weight75 kDa
GenBank accession numberBC021892
Gene Symbol SCARB2
Gene ID (NCBI) 950
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ14108
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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