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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IMUP Recombinant antibody (84148-7-RR)

    WB analysis using 84148-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84148-7-RR (IMUP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A431 cells using IMUP Recombinant antibody (84148-7-RR)

    FC experiment of A431 using 84148-7-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug IMUP Recombinant antibody (84148-7-RR, Clone:241220A4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Flow cytometry (FC) experiment of PC-3 cells using IMUP Recombinant antibody (84148-7-RR)

    FC experiment of PC-3 using 84148-7-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug IMUP Recombinant antibody (84148-7-RR, Clone:241220A4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

IMUP Recombinant monoclonal antibody

IMUP Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84148-7-RR
Clone No.241220A4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

241220A4, C19orf33, H2RSP, HAI-2-related small protein, Hepatocyte growth factor activator inhibitor type 2-related small protein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, L02 cells, A549 cells, EC109 cells
Positive FC (Intra) detected inA431 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84148-7-RR targets IMUP in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag22218

Product name: Recombinant human C19orf33 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-85 aa of BC060319

Sequence: MEFDLGAALEPTSQKPGVGAGHGGDPKLSPHKVQGRSEAGAGPGPKASNFRGLGKGRRLTAAPPSSKDTTALPTPAAAPAIRTRM

相同性解析による交差性が予測される生物種
Full Name chromosome 19 open reading frame 33
Calculated molecular weight 106 aa, 11 kDa
Observed molecular weight20-22 kDa
GenBank accession numberBC060319
Gene Symbol IMUP
Gene ID (NCBI) 64073
RRIDAB_3671712
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9GZP8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Immortalization up-regulated protein (IMUP), also known as C19orf33 or H2RSP, was first identified in SV40-immortalized fibroblasts and includes two isoforms, IMUP-1 and IMUP-2 (PMID: 14981917; 35142956). It is found primarily in the nucleus (PMID: 11606055). IMUP is reportedly upregulated in many cancer cells and tissues and its overexpression is associated with tumorigenicity and cell-cycle acceleration (PMID: 16962562; 35142956; 22886722). The apparent molecular determined by SDS-PAGE might be due to post-translational modifications or anomalous migration behavior (PMID: 11080599).

Protocols

Product Specific Protocols
WB protocol for IMUP antibody 84148-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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