• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using NELF-B Recombinant antibody (84135-4-RR)

    WB analysis using 84135-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84135-4-RR (NELF-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using NELF-B Recombinant antibody (84135-4-RR)

    IF Staining of A431 using 84135-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NELF-B antibody (84135-4-RR, Clone: 241434F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A431 cells using NELF-B Recombinant antibody (84135-4-RR)

    FC experiment of A431 using 84135-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug NELF-B Recombinant antibody (84135-4-RR, Clone:241434F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Flow cytometry (FC) experiment of BxPC-3 cells using NELF-B Recombinant antibody (84135-4-RR)

    FC experiment of BxPC-3 using 84135-4-RR

    1x10^6 BxPC-3 cells were intracellularly stained with 0.25 ug NELF-B Recombinant antibody (84135-4-RR, Clone:241434F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84135-4-RR

    Affinity and Kinetic Characterization of 84135-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84135-4-RR against Human NELF-B were performed. The affinity constant is 0.199 nM.

NELFB Recombinant monoclonal antibody

NELFB Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84135-4-RR
Clone No.241434F12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

NELF B, Negative elongation factor B, KIAA1182, Cofactor of BRCA1, COBRA1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, BxPC-3 cells, A431 cells
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells, BxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84135-4-RR targets NELFB in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag9599

Product name: Recombinant human NELF-B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 127-475 aa of BC011892

Sequence: VHYCTLRAELLMSLHDLDVGEICTVDPCHKFTWCLDACIRERFVDSKRARELQGFLDGVKKGQEQVLGDLSMILCDPFAINTLALSTVRHLQELVGQETLPRDSPDLLLLLRLLALGQGAWDMIDSQVFKEPKMEVELITRFLPMLMSFLVDDYTFNVDQKLPAEEKAPVSYPNTLPESFTKFLQEQRMACEVGLYYVLHITKQRNKNALLRLLPGLVETFGDLAFGDIFLHLLTGNLALLADEFALEDFCSSLFDGFFLTASPRKENVHRHALRLLIHLHPRVAPSKLEALQKALEPTGQSGEAVKELYSQLGEKLEQLDHRKPSPAQAAETPALELPLPSVPAPAPL

相同性解析による交差性が予測される生物種
Full Name cofactor of BRCA1
Calculated molecular weight580aa,66 kDa; 475aa,54 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC011892
Gene Symbol NELFB
Gene ID (NCBI) 25920
RRIDAB_3671694
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ8WX92
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cofactor of BRCA1 (COBRA1) was first identified as a BRCA1-interacting protein and subsequently found to be the B subunit of the negative elongation factor complex (NELF-B). Cobra1 is essential for early embryogenesis (PMID: 19340312). The MW of this protein is 66 kDa, and this antibody specially recognises the 66 kDa protein.

Protocols

Product Specific Protocols
WB protocol for NELFB antibody 84135-4-RRDownload protocol
IF protocol for NELFB antibody 84135-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols