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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using CD98 Recombinant antibody (84040-7-RR)

    WB analysis of mouse kidney using 84040-7-RR

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 84040-7-RR (CD98 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using CD98 Recombinant antibody (84040-7-RR)

    WB analysis of mouse testis using 84040-7-RR

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 84040-7-RR (CD98 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CD98 Recombinant antibody (84040-7-RR)

    IHC staining of mouse kidney using 84040-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84040-7-RR (CD98 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CD98 Recombinant antibody (84040-7-RR)

    IHC staining of mouse kidney using 84040-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84040-7-RR (CD98 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CD98 Recombinant antibody (84040-7-RR)

    IHC staining of mouse brain using 84040-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84040-7-RR (CD98 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CD98 Recombinant antibody (84040-7-RR)

    IHC staining of mouse brain using 84040-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84040-7-RR (CD98 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84040-7-RR

    Affinity and Kinetic Characterization of 84040-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84040-7-RR against Mouse CD98/SLC3A2 were performed. The affinity constant is 1.36 nM.

CD98/SLC3A2 Recombinant monoclonal antibody

CD98/SLC3A2 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 84040-7-RR
Clone No.241247F12

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

4F2 cell-surface antigen heavy chain, 4F2hc, Amino acid transporter heavy chain SLC3A2, CD98, Mdu1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse kidney tissue, mouse testis tissue
Positive IHC detected inmouse brain tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84040-7-RR targets CD98/SLC3A2 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg1374

Product name: Recombinant Mouse CD98 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: N-6*His

Domain: 100-526 aa of NM_008577.4

Sequence: APRCRELPVQRWWHKGALYRIGDLQAFVGRDAGGIAGLKSHLEYLSTLKVKGLVLGPIHKNQKDEINETDLKQINPTLGSQEDFKDLLQSAKKKSIHIILDLTPNYQGQNAWFLPAQADIVATKMKEALSSWLQDGVDGFQFRDVGKLMNAPLYLAEWQNITKNLSEDRLLIAGTESSDLQQIVNILESTSDLLLTSSYLSNSTFTGERTESLVTRFLNATGSQWCSWSVSQAGLLADFIPDHLLRLYQLLLFTLPGTPVFSYGDELGLQGALPGQPAKAPLMPWNESSIFHIPRPVSLNMTVKGQNEDPGSLLTQFRRLSDLRGKERSLLHGDFHALSSSPDLFSYIRHWDQNERYLVVLNFRDSGRSARLGASNLPAGISLPASAKLLLSTDSARQSREEDTSLKLENLSLNPYEGLLLQFPFVA

相同性解析による交差性が予測される生物種
Full Name solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
Calculated molecular weight58 kDa
GenBank accession numberNM_008577.4
Gene Symbol CD98/SLC3A2
Gene ID (NCBI) 17254
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP10852-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).

Protocols

Product Specific Protocols
IHC protocol for CD98/SLC3A2 antibody 84040-7-RRDownload protocol
WB protocol for CD98/SLC3A2 antibody 84040-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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