Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84035-3-RR targets UTP18 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag34662 Product name: Recombinant human UTP18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 72-205 aa of NM_016001 Sequence: RNRLRLEEDKPAVERCLEELVFGDVENDEDALLRRLRGPRVQEHEDSGDSEVENEAKGNFPPQKKPVWVDEEDEDEEMVDMMNNRFRKDMMKNASESKLSKDNLKKRLKEEFQHAMGGVPAWAETTKRKTS 相同性解析による交差性が予測される生物種 |
| Full Name | UTP18, small subunit (SSU) processome component, homolog (yeast) |
| Calculated molecular weight | 62 kDa |
| Observed molecular weight | 62 kDa |
| GenBank accession number | NM_016001 |
| Gene Symbol | UTP18 |
| Gene ID (NCBI) | 51096 |
| RRID | AB_3671604 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9Y5J1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UTP18 (UTP18 small subunit processome component), also known as WDR50. It is expected to be located in nucleus. The protein is one part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Involved in nucleolar processing of pre-18S ribosomal RNA. The calculated molecular weight of UTP18 is 62 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for UTP18 antibody 84035-3-RR | Download protocol |
| WB protocol for UTP18 antibody 84035-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
