Validation Data Gallery
Tested Applications
| Positive WB detected in | SCF treated Mo7e cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83925-1-RR targets Phospho-CD117/c-Kit (Tyr703) in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog |
| Calculated molecular weight | 976 aa, 110 kDa |
| Observed molecular weight | 140-160 kDa |
| GenBank accession number | BC071593 |
| Gene Symbol | CD117 |
| Gene ID (NCBI) | 3815 |
| RRID | AB_3671505 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P10721 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Kit is composed of five N-glycosylated immunoglobulin domains in the N-terminal extracellular portion that bind SCF, a transmembrane domain, and the carboxy-terminal intracellular tyrosine kinase domain. The binding of SCF autophosphorylates Kit on specific tyrosine residues (Tyr), such as Tyr568/570, Tyr703, Tyr721, and Tyr936. Kit then binds to other cytoplasmic enzymes, such as PI3K and Src kinases, and this complex activates downstream molecules. This activates the Akt-Bad pathway and the Ras-Mek-Erk cascade, which regulate gene expression and cytoskeletal structures, resulting in cell proliferation and survival. (PMID: 28403213)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-CD117/c-Kit (Tyr703) antibody 83925-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |