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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using ANK2 Recombinant antibody (83912-5-RR)

    IHC staining of mouse cerebellum using 83912-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83912-5-RR (ANK2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using ANK2 Recombinant antibody (83912-5-RR)

    IHC staining of mouse cerebellum using 83912-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83912-5-RR (ANK2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using ANK2 Recombinant antibody (83912-5-RR)

    IF Staining of mouse heart using 83912-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using ANK2 antibody (83912-5-RR, Clone: 240982G5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using ANK2 Recombinant antibody (83912-5-RR)

    IF Staining of mouse heart using 83912-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using ANK2 antibody (83912-5-RR, Clone: 240982G5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HEK-293 cells using ANK2 Recombinant antibody (83912-5-RR)

    FC experiment of HEK-293 using 83912-5-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Ank2 Recombinant Antibody (83912-5-RR, Clone:240982G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83912-5-RR

    Affinity and Kinetic Characterization of 83912-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83912-5-RR against Human ANK2 were performed. The affinity constant is below 1 pM.

ANK2 Recombinant monoclonal antibody

ANK2 Uni-rAb® Recombinant Antibody for IHC, IF-P, FC (Intra), ELISA
Cat No. 83912-5-RR
Clone No.240982G5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, FC (Intra), ELISA

Synonyms

240982G5, ANK 2, Ankyrin 2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83912-5-RR targets ANK2 in IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34642

Product name: Recombinant human ANK2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 3571-3708 aa of NM_001148

Sequence: ERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGKHATDTNLVECLTKINRMDIVHLMETNTEPLQERISHSYAEIEQTITLDHSEGFSVLQEELCTAQHKQKEEQAVSKESETCDH

相同性解析による交差性が予測される生物種
Full Name ankyrin 2, neuronal
Calculated molecular weight434 kDa
Observed molecular weight250-260 kDa
GenBank accession numberNM_001148
Gene Symbol ANK2
Gene ID (NCBI) 287
RRIDAB_3671493
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ01484
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Ankyrins, belonging to the adapter protein family, consist of Ankyrin-R (ANK1), Ankyrin-B (ANK2), and Ankyrin-G (ANK3). Initially, ANK2 was considered as a key cytoskeletal-associated protein in the brain. Besides, it was discovered as a pivotal adaptor and scaffolding protein in the heart. Remarkably, recent studies have demonstrated that Ankyrins contribute to the modulation of various biological activities in cancers. (PMID: 36539782)

Protocols

Product Specific Protocols
FC protocol for ANK2 antibody 83912-5-RRDownload protocol
IF protocol for ANK2 antibody 83912-5-RRDownload protocol
IHC protocol for ANK2 antibody 83912-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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