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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD83 Recombinant monoclonal antibody (83907-5-RR)

    WB analysis using 83907-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83907-5-RR (CD83 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using CD83 Recombinant monoclonal antibody (83907-5-RR)

    WB analysis of mouse spleen using 83907-5-RR

    Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 83907-5-RR (CD83 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

CD83 Recombinant monoclonal antibody

CD83 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 83907-5-RR
Clone No.252734F1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

CD83 antigen, mCD83

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRaji cells, mouse spleen tissue, mouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83907-5-RR targets CD83 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg10435

Product name: Recombinant mouse CD83 protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 10011478 of

Sequence: \

相同性解析による交差性が予測される生物種
Full Name CD83 antigen
Observed molecular weight50 kDa
Gene Symbol Cd83
Gene ID (NCBI) 12522
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO88324
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also be expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928). PNGase F (peptide N-glycosidase F) digestion reduced the 37 kDa and 50 kDa CD83 forms to 28 kDa (PMID: 15320871).

Protocols

Product Specific Protocols
WB protocol for CD83 antibody 83907-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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