Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83900-7-RR targets KIAA1586 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag36038 Product name: Recombinant human KIAA1586 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 426-749 aa of BC041125 Sequence: SLDDSISEIKQINHLKIFIDKIYSIYHQPNKNQTKLLGTVAKELETEIIKIGRVMGPRWAACSLQAATAVWHAYPILYMHFSHSYSGLAKRLANINFLQDLALMIDILEEFSVLSTALQSRSTNIKKAQKLIKRTIRALENLKIGTGKYESQIEDLIKSDKFKDIPFNKNNKFNALPRSILLDNIIQHMNLRLLSDRNHEDIFNYFDLLEPSTWPYEEITSPWIAGEKTLFHLCKILKYEVDLNDFREFVNNNIKSNNVSIPTTIYKAKKIVSTIAINSAEAERGFNLMNIICTRVRNSLTIDHVSDLMTINLLGKELADWDAT 相同性解析による交差性が予測される生物種 |
| Full Name | KIAA1586 |
| Calculated molecular weight | 787 aa, 90 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC041125 |
| Gene Symbol | KIAA1586 |
| Gene ID (NCBI) | 57691 |
| RRID | AB_3671481 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q9HCI6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KIAA1586, close to ZNF451, encodes a protein with similar N-terminal tandem SIMs, which has also shown elongase activity. ZNF451 is an E3 SUMO-protein ligase that facilitates UBE2I/UBC9-mediated SUMO2 modification of target proteins (PMID: 26524493, PMID: 35887358).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KIAA1586 antibody 83900-7-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |