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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Tissue Factor/CD142 Recombinant antibody (83887-4-RR)

    WB analysis using 83887-4-RR (same clone as 83887-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83887-4-RR (Coagulation Factor III/Tissue Factor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83887-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using Tissue Factor/CD142 Recombinant antibody (83887-4-RR)

    WB analysis of mouse brain using 83887-4-RR (same clone as 83887-4-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 83887-4-RR (Coagulation Factor III/Tissue Factor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83887-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00859-1

    Sandwich ELISA standard curve of MP00859-1

    Sandwich ELISA standard curve of MP00859-1, Mouse Tissue Factor Recombinant Matched Antibody Pair - PBS only. 83887-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1102. 83887-4-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL.

  • Sample test of MP00859-1

    Sample test of MP00859-1

    Urine of mice was measured. The Tissue Factor concentration of detected samples was determined to be 244.1 pg/mL.

  • Sample test of MP00859-1

    Sample test of MP00859-1

    The mean Tissue Factor concentration was determined to be 18.4 ng/mL in mouse brain tissue extract based on a 6.0 mg/mL extract load, 13.1 ng/mL in C2C12 cell extract based on a 3.6 mg/mL extract load and 2.7 ng/mL in NIH/3T3 cell extract based on a 10.6 mg/mL extract load.

  • Sample test of MP00859-1

    Sample test of MP00859-1

    Serum of sixteen mice was measured. The Tissue Factor concentration of detected samples was determined to be 86.6 pg/mL with a range of 63.0-105.0 pg/mL.

  • Cytometric bead array standard curve of MP00859-1

    Cytometric bead array standard curve of MP00859-1

    Cytometric bead array standard curve of MP00859-1, MOUSE Tissue Factor Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83887-1-PBS. Detection antibody: 83887-4-PBS. Standard: Eg1102. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83887-4-RR

    Affinity and Kinetic Characterization of 83887-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83887-4-RR against Mouse Tissue Factor were performed. The affinity constant is 0.85 nM.

Mouse Tissue Factor/CD142 Recombinant monoclonal antibody, PBS Only (Detector)

Tissue Factor/CD142 Uni-rAb® Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83887-4-PBS
Clone No.240875H3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CD142, Coagulation factor III, F3, Tissue factor, 240875H3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83887-4-PBS targets Tissue Factor/CD142 as part of a matched antibody pair:

MP00859-1: 83887-1-PBS capture and 83887-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg1102

Product name: Recombinant Mouse Tissue Factor protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 29-251 aa of

Sequence: AGIPEKAFNLTWISTDFKTILEWQPKPTNYTYTVQISDRSRNWKNKCFSTTDTECDLTDEIVKDVTWAYEAKVLSVPRRNSVHGDGDQLVIHGEEPPFTNAPKFLPYRDTNLGQPVIQQFEQDGRKLNVVVKDSLTLVRKNGTFLTLRQVFGKDLGYIITYRKGSSTGKKTNITNTNEFSIDVEEGVSYCFFVQAMIFSRKTNQNSPGSSTVCTEQWKSFLGE

相同性解析による交差性が予測される生物種
Full Name coagulation factor III
Calculated molecular weight33kDa
Observed molecular weight48-53 kDa
Gene Symbol Tissue Factor
Gene ID (NCBI) 14066
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP20352
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named Coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108). This antibody is specific for mouse Tissue Factor.

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