Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83880-5-RR targets LRFN5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag22152 Product name: Recombinant human LRFN5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 579-719 aa of BC043165 Sequence: QGCSVTLPQSVSKQAVGHEENAQCCKATSDNVIQSSETCSSQDSSTTTSALPPSWTSSTSVSQKQKRKTGTKPSTEPQNEAVTNVESQNTNRNNSTALQLASRPPDSVTEGPTSKRAHIKPNALLTNVDQIVQETQRLELI 相同性解析による交差性が予測される生物種 |
| Full Name | leucine rich repeat and fibronectin type III domain containing 5 |
| Calculated molecular weight | 719 aa, 79 kDa |
| Observed molecular weight | 79 kDa |
| GenBank accession number | BC043165 |
| Gene Symbol | LRFN5 |
| Gene ID (NCBI) | 145581 |
| RRID | AB_3671463 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q96NI6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Leucine-rich repeat and fibronectin type-III domain-containing protein 5(LRFN5), belongs to a family of five small transmembrane protein genes involved in the development, organization, and plasticity of synapses. LRFN5 has another synonym which stands for synaptic adhesion-like molecule 5 (SALM5). SALMs are newly characterized adhesion molecules predominantly expressed in the brain contributing to neurite outgrowth and synapse formation. (PMID: 37280213)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for LRFN5 antibody 83880-5-RR | Download protocol |
| WB protocol for LRFN5 antibody 83880-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |