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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VEGF-C Recombinant monoclonal antibody (83727-7-RR)

    WB analysis using 83727-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83727-7-RR (VEGF-C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using VEGF-C Recombinant monoclonal antibody (83727-7-RR)

    IF Staining of MCF-7 using 83727-7-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using VEGF-C antibody (83727-7-RR, Clone: 251992H1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Affinity and Kinetic Characterization of 83727-7-RR

    Affinity and Kinetic Characterization of 83727-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83727-7-RR against Human VEGF-C were performed. The affinity constant is below 1 pM.

VEGF-C Recombinant monoclonal antibody

VEGF-C Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83727-7-RR
Clone No.251992H1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

VEGFC, vascular endothelial growth factor C, Vascular endothelial growth factor-related protein, VEGF C

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, SW480 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83727-7-RR targets VEGF-C in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg1482

Product name: Recombinant human VEGFC protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 112-227 aa of BC035212

Sequence: AHYNTEILKSIDNEWRKTQCMPREVCIDVGKEFGVATNTFFKPPCVSVYRCGGCCNSEGLQCMNTSTSYLSKTLFEITVPLSQGPKPVTISFANHTSCRCMSKLDVYRQVHSIIRR

相同性解析による交差性が予測される生物種
Full Name vascular endothelial growth factor C
Calculated molecular weight 419 aa, 47 kDa
Observed molecular weight43 kDa
GenBank accession numberBC035212
Gene Symbol VEGFC
Gene ID (NCBI) 7424
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP49767
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VEGFC is a member of the platelet-derived growth factor / vascular endothelial growth factor (PDGF/VEGF) family. The main function of VEGFC is to promote the growth of lymphatic vessels (lymphangiogenesis). It acts on lymphatic endothelial cells (LECs) primarily via its receptor VEGFR-3 promoting survival, growth, and migration.

Protocols

Product Specific Protocols
IF protocol for VEGF-C antibody 83727-7-RRDownload protocol
WB protocol for VEGF-C antibody 83727-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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