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Validation Data Gallery

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  • Western Blot (WB) analysis of 3T3-L1 cells using VCAM-1/CD106 Recombinant antibody (83719-1-RR)

    WB analysis of 3T3-L1 using 83719-1-RR (same clone as 83719-1-PBS)

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 83719-1-RR (VCAM-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83719-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00701-4

    Sandwich ELISA standard curve of MP00701-4

    Sandwich ELISA standard curve of MP00701-4, Mouse VCAM-1/CD106 Recombinant Matched Antibody Pair - PBS only. 83719-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0639. 83719-3-PBS was HRP conjugated as the detection antibody. Range: 46.88-3000 pg/mL.

  • Sample test of MP00701-4

    Sample test of MP00701-4

    Serum of eight mice was measured. The VCAM-1 concentration of detected samples was determined to be 572.76 ng/mL with a range of 483.18-844.96 ng/mL.

  • Sample test of MP00701-4

    Sample test of MP00701-4

    Urine of eight mice was measured. The VCAM-1 concentration of detected samples was determined to be 71.97 ng/mL with a range of 8.65-105.58 ng/mL.

  • Sample test of MP00701-4

    Sample test of MP00701-4

    Mouse lung, cut into 1-2 mm pieces, was cultured for 3 days in 25-30 mL of RPMI supplemented with 10% fetal bovine serum and unstimulated or stimulated with 10 μg/mL ConA. The mean VCAM-1 concentration was determined to be 14.2 ng/mL in unstimulated mouse lung superantant, 20.9 ng/mL in ConA stimulated mouse lung superantant.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using VCAM-1/CD106 Recombinant antibody (83719-1-RR)

    IHC staining of mouse spleen using 83719-1-RR (same clone as 83719-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83719-1-RR (VCAM-1/CD106 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83719-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using VCAM-1/CD106 Recombinant antibody (83719-1-RR)

    IHC staining of mouse spleen using 83719-1-RR (same clone as 83719-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83719-1-RR (VCAM-1/CD106 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83719-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83719-1-RR

    Affinity and Kinetic Characterization of 83719-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83719-1-RR against Mouse VCAM-1 were performed. The affinity constant is 0.383 nM.

Mouse VCAM-1/CD106 Recombinant monoclonal antibody, PBS Only (Capture)

VCAM-1/CD106 Uni-rAb® Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83719-1-PBS
Clone No.240597B2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CD106, Vcam1, VCAM-1, 240597B2, Vcam 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83719-1-PBS targets VCAM-1/CD106 as part of a matched antibody pair:

MP00701-4: 83719-1-PBS capture and 83719-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0639

Product name: Recombinant mouse VCAM-1 protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 25-698 aa of CAA47989.1

Sequence: FKIEISPEYKTIAQIGDSMALTCSTTGCESPLFSWRTQIDSPLNAKVRTEGSKSVLTMEPVSFENEHSYLCTATCGSGKLERSIHVDIYSFPKDPEIQFSGPLEVGKPVTVKCLAPDIYPVYRLEIDLFKGDQLMNRQEFSSEEMTKSLETKSLEVTFTPVIEDIGKALVCRAKLHIDQIDSTLKERETVKELQVYISPRNTTISVHPSTRLQEGGAVTMTCSSEGLPAPEIFWGRKLDNEVLQLLSGNATLTLIAMRMEDSGVYVCEGVNLIGRDKAEVELVVQEKPFIVDISPGSQVAAQVGDSVVLTCAAIGCDSPSFSWRTQTDSPLNGVVRNEGAKSTLVLSSVGFEDEHSYLCAVTCLQRTLEKRTQVEVYSFPEDPVIKMSGPLVHGRPVTVNCTVPNVYPFDHLEIELLKGETTLMKKYFLEEMGIKSLETKILETTFIPTIEDTGKSLVCLARLHSGEMESEPKQRQSVQPLYVNVAPKETTIWVSPSPILEEGSPVNLTCSSDGIPAPKILWSRQLNNGELQPLSENTTLTFMSTKRDDSGIYVCEGINEAGISRKSVELIIQVSPKDIQLTVFPSKSVKEGDTVIISCTCGNVPETWIILKKKAKTGDMVLKSVDGSYTIRQAQLQDAGIYECESKTEVGSQLRSLTLDVKGKEHNKDYFSPE

相同性解析による交差性が予測される生物種
Full Name vascular cell adhesion molecule 1
Calculated molecular weight81 kDa
Observed molecular weight90 kDa
GenBank accession numberCAA47989.1
Gene Symbol VCAM-1
Gene ID (NCBI) 22329
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP29533
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

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