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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSP47/SERPINH1 Recombinant antibody (83689-4-RR)

    WB analysis using 83689-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83689-4-RR (HSP47/SERPINH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HSP47/SERPINH1 Recombinant monoclonal antibody (83689-4-RR)

    WB analysis of HeLa using 83689-4-RR

    WB result of HSP47/SERPINH1 antibody (83689-4-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP47/SERPINH1 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using HSP47/SERPINH1 Recombinant antibody (83689-4-RR)

    IHC staining of human ovarian cancer using 83689-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83689-4-RR (HSP47/SERPINH1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HSP47/SERPINH1 Recombinant antibody (83689-4-RR)

    IF Staining of HeLa using 83689-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP47/SERPINH1 antibody (83689-4-RR, Clone: 240752F2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HSP47/SERPINH1 Recombinant antibody (83689-4-RR)

    IF Staining of HeLa using 83689-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP47/SERPINH1 antibody (83689-4-RR, Clone: 240752F2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83689-4-RR

    Affinity and Kinetic Characterization of 83689-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83689-4-RR against Human HSP47/SERPINH1 were performed. The affinity constant is 5.99 nM.

  • Featured Product
  • KD/KO Validated

HSP47/SERPINH1 Recombinant monoclonal antibody

HSP47/SERPINH1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83689-4-RR
Clone No.240752F2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

HSP47, SERPINH1, 240752F2, 47 kDa heat shock protein, Arsenic-transactivated protein 3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA2780 cells, HeLa cells, A549 cells, HT-1080 cells, MCF-7 cells, HepG2 cells, NIH/3T3 cells
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83689-4-RR targets HSP47/SERPINH1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg1201

Product name: Recombinant Human HSP47/SERPINH1 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 19-418 aa of NM_001235.5

Sequence: AEVKKPAAAAAPGTAEKLSPKAATLAERSAGLAFSLYQAMAKDQAVENILVSPVVVASSLGLVSLGGKATTASQAKAVLSAEQLRDEEVHAGLGELLRSLSNSTARNVTWKLGSRLYGPSSVSFADDFVRSSKQHYNCEHSKINFRDKRSALQSINEWAAQTTDGKLPEVTKDVERTDGALLVNAMFFKPHWDEKFHHKMVDNRGFMVTRSYTVGVMMMHRTGLYNYYDDEKEKLQIVEMPLAHKLSSLIILMPHHVEPLERLEKLLTKEQLKIWMGKMQKKAVAISLPKGVVEVTHDLQKHLAGLGLTEAIDKNKADLSRMSGKKDLYLASVFHATAFELDTDGNPFDQDIYGREELRSPKLFYADHPFIFLVRDTQSGSLLFIGRLVRPKGDKMRDEL

相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Calculated molecular weight46kDa
Observed molecular weight 46 kDa
GenBank accession numberNM_001235.5
Gene Symbol HSP47/SERPINH1
Gene ID (NCBI) 871
RRIDAB_3671292
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP50454
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSP47 is also named as Serpin H1, colligen, CBP1, CBP2, HSP47, SERPINH2 and belongs to the serpin family. This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum. It binds specifically to collagen and can be involved as a chaperone in the biosynthetic pathway of collagen. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. HSP47 has a calculated molecular weight of 46 kDa.

Protocols

Product Specific Protocols
IF protocol for HSP47/SERPINH1 antibody 83689-4-RRDownload protocol
IHC protocol for HSP47/SERPINH1 antibody 83689-4-RRDownload protocol
WB protocol for HSP47/SERPINH1 antibody 83689-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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