Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, Caco-2 cells, COS-7 cells, mouse brain tissue, rat brain tissue |
| Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83618-5-RR targets Presenilin-1-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
| Tested Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | presenilin 1 |
| Calculated molecular weight | 467 aa, 53 kDa |
| Observed molecular weight | 21 kDa, 53 kDa |
| GenBank accession number | BC011729 |
| Gene Symbol | PSEN1 |
| Gene ID (NCBI) | 5663 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P49768 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PSEN1(presenilin-1) is also named as AD3, PS1, PSNL1 and belongs to the peptidase A22A family. It directly influences Netrin/DCC signaling with cell type precision and spatiotemporal specificity, and is a key neural circuit builder that gates the spatiotemporal pattern of guidance signaling, thereby ensuring neural projections occur with high fidelity(PMID:21215373). The 46-kDa PSEN1 holoprotein is cleaved into a D30-kDa N-terminal fragment (NTF) and a D20-kDa C-terminal fragment (CTF) by the 26S proteasome (PMID:10527805). It has 7 isoforms produced by alternative splicing. This antibody is specific to isoform1(53 kDa), isoform2(52 kDa), isoform4(21 kDa), isoform7(49 kDa) of this protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Presenilin-1-Specific antibody 83618-5-RR | Download protocol |
| WB protocol for Presenilin-1-Specific antibody 83618-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |