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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPSN2 Recombinant antibody (83592-4-RR)

    WB analysis using 83592-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83592-4-RR (GPSN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using GPSN2 Recombinant antibody (83592-4-RR)

    IF Staining of U-251 using 83592-4-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using GPSN2 antibody (83592-4-RR, Clone: 240472D4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using GPSN2 Recombinant antibody (83592-4-RR)

    IF Staining of U-251 using 83592-4-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using GPSN2 antibody (83592-4-RR, Clone: 240472D4 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  • Flow cytometry (FC) experiment of U-251 cells using GPSN2 Recombinant antibody (83592-4-RR)

    FC experiment of U-251 using 83592-4-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug GPSN2 Recombinant antibody (83592-4-RR, Clone:240472D4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

GPSN2 Recombinant monoclonal antibody

GPSN2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83592-4-RR
Clone No.240472D4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

240472D4, EC:1.3.1.93, SC2, Synaptic glycoprotein SC2, TECR

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, human placenta tissue
Positive IF/ICC detected inU-251 cells
Positive FC (Intra) detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83592-4-RR targets GPSN2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag32598

Product name: Recombinant human GPSN2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-87 aa of BC002425

Sequence: MKHYEVEILDAKTREKLCFLDKVEPHATIAEIKNLFTKTHPQWYPARQSLRLDPKGKSLKDEDVLQKLPVGTTATLYFRDLGAQISW

相同性解析による交差性が予測される生物種
Full Name glycoprotein, synaptic 2
Calculated molecular weight 36 kDa
Observed molecular weight32-36 kDa
GenBank accession numberBC002425
Gene Symbol GPSN2
Gene ID (NCBI) 9524
RRIDAB_3671207
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9NZ01
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glycoprotein synaptic 2 (GPSN2), also known as TER and TECR, functions as a trans-2,3-enoyl-CoA reductase and reduces long and very long chain fatty acid trans-2,3-enoyl-CoA substrates (PMID: 12482854).

Protocols

Product Specific Protocols
IF protocol for GPSN2 antibody 83592-4-RRDownload protocol
WB protocol for GPSN2 antibody 83592-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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