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Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00533-3

    Sandwich ELISA standard curve of MP00533-3

    Sandwich ELISA standard curve of MP00533-3, mouse IL-2 Recombinant Matched Antibody Pair - PBS only. 98055-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0762. 83547-1-PBS was HRP conjugated as the detection antibody. Range: 6.25-400 pg/mL.

  • Sample test of MP00533-3

    Sample test of MP00533-3

    EL-4 mouse lymphoblast cells (1 x 10^6  cells/mL) were cultured for 3 days in DMEM supplemented with 10% fetal bovine serum and stimulated with 10 μg/mL PHA and 10 ng/mL PMA. The mean mouse IL-2 concentration was determined to be 16.1 pg/mL in unstimulated EL-4, 309.9 ng/mL in PHA and PMA stimulated EL-4 supernatant.

  • Sample test of MP00533-3

    Sample test of MP00533-3

    Serum of sixteen mice was measured. were measured. The mouse IL-2 concentration was determined to be 2.27 pg/mL with a range of 1.1-79.9 pg/mL.

  • Affinity and Kinetic Characterization of 83547-1-PBS

    Affinity and Kinetic Characterization of 83547-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 83547-1-PBS against Mouse IL-2 were performed. The affinity constant is 3.03 nM.

Mouse IL-2 Recombinant monoclonal antibody, PBS Only (Detector)

IL-2 Uni-rAb® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83547-1-PBS
Clone No.240358A4

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Il2, Il 2, interleukin 2, Interleukin-2, T-cell growth factor

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83547-1-PBS targets IL-2 as part of a matched antibody pair:

MP00533-3: 98055-1-PBS capture and 83547-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0762

Product name: Recombinant Mouse IL-2 protein (His Tag)

Source: mammalian cells-derived, pCMV (S9)

Tag: N-6*His

Domain: 21-169 aa of NM_008366

Sequence: APTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFCQSIISTSPQ

相同性解析による交差性が予測される生物種
Full Name interleukin 2
Calculated molecular weight 19 kDa
GenBank accession number NM_008366
Gene Symbol IL-2
Gene ID (NCBI) 16183
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP04351
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.