Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, K-562 cells, TT cells, A549 cells |
| Positive FC (Intra) detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83363-1-RR targets RBM20 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag34322 Product name: Recombinant human RBM20 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 667-840 aa of NM_001134363 Sequence: ADWGNGRDSWEHSPYARREEERDPAPWRDNGDDKRDRMDPWAHDRKHHPRQLDKAELDERPEGGRPHREKYPRSGSPNLPHSVSSYKSREDGYYRKEPKAKSDKYLKQQQDAPGRSRRKDEARLRESRHPHPDDSGKEDGLGPKVTRAPEGAKAKQNEKNKTKRTDRDQEGADD 相同性解析による交差性が予測される生物種 |
| Full Name | RNA binding motif protein 20 |
| Calculated molecular weight | 134 kDa |
| Observed molecular weight | 190 kDa |
| GenBank accession number | NM_001134363 |
| Gene Symbol | RBM20 |
| Gene ID (NCBI) | 282996 |
| RRID | AB_3671017 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q5T481 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RBM20 (RNA binding motif protein 20) is a heart- and skeletal muscle- nriched splicing factor that controls tissue-specific isoform expression of many other genes besides TTN, including sarcomeric genes such myomesin 1, but also Ca2+- and ion-handling genes such as CAMK2D (calcium/calmodulin-dependent kinase II-delta) and RYR2 (ryanodine receptor 2) (PMID: 29650543).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RBM20 antibody 83363-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |