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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FGR Recombinant antibody (83352-1-RR)

    WB analysis using 83352-1-RR (same clone as 83352-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83352-1-RR (FGR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83352-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00370-1

    Sandwich ELISA standard curve of MP00370-1

    Sandwich ELISA standard curve of MP00370-1, Human FGR Recombinant Matched Antibody Pair - PBS only. 83352-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34944. 83352-3-PBS was HRP conjugated as the detection antibody. Range: 0.0781-25 ng/mL

  • Sample test of MP00370-1

    Sample test of MP00370-1

    Raji cell lysate was measured. The human FGR concentration of detected samples was determined to be 23.90 ng/mL (based on a 1.2 mg/mL extract load).

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using FGR Recombinant antibody (83352-1-RR)

    IF Staining of Raji using 83352-1-RR (same clone as 83352-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using FGR antibody (83352-1-RR, Clone: 240275E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83352-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Raji cells using FGR Recombinant antibody (83352-1-RR)

    FC experiment of Raji using 83352-1-RR (same clone as 83352-1-PBS)

    1x10^6 Raji cells were intracellularly stained with 0.25 ug FGR Recombinant antibody (83352-1-RR, Clone:240275E5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83352-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00370-1

    Cytometric bead array standard curve of MP00370-1

    Cytometric bead array standard curve of MP00370-1, FGR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83352-1-PBS. Detection antibody: 83352-3-PBS. Standard: Ag34944. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83352-1-PBS

    Affinity and Kinetic Characterization of 83352-1-PBS

    Biolayer interferometry (BLl) kinetic assay of 83352-1-PBS against Human FGR was performed. The affinity constant is 28.2 pM.

  • Affinity and Kinetic Characterization of 83352-1-RR

    Affinity and Kinetic Characterization of 83352-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83352-1-RR against Human FGR were performed. The affinity constant is 0.139 nM.

FGR Recombinant monoclonal antibody, PBS Only (Capture)

FGR Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83352-1-PBS
Clone No.240275E5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

p58c fgr, p55c fgr, p55 Fgr, c src2, c fgr

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83352-1-PBS targets FGR as part of a matched antibody pair:

MP00370-1: 83352-1-PBS capture and 83352-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34944

Product name: Recombinant human FGR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-90 aa of NM_005248

Sequence: MGCVFCKKLEPVATAKEDAGLEGDFRSYGAADHYGPDPTKARPASSFAHIPNYSNFSSQAINPGFLDSGTIRGVSGIGVTLFIALYDYEA

相同性解析による交差性が予測される生物種
Full Name Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog
Calculated molecular weight59 kDa
Observed molecular weight60 kDa
GenBank accession numberNM_005248
Gene Symbol FGR
Gene ID (NCBI) 2268
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP09769
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FGR (Tyrosine-protein kinase Fgr) is a member of the Src family of protein tyrosine kinases (PTKs). It's contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively.