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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SAT1 Recombinant monoclonal antibody (83319-1-RR)

    WB analysis using 83319-1-RR (same clone as 83319-1-PBS)

    Untreated and DENSPM treated HEK-293 and HeLa cells were subjected to SDS PAGE followed by western blot with 83319-1-RR (SAT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83319-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using SAT1 Recombinant monoclonal antibody (83319-1-RR)

    WB analysis of K-562 using 83319-1-RR (same clone as 83319-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83319-1-RR (SAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83319-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP00344-4

    Sandwich ELISA sample test of MP00344-4

    The mean SAT1 concentration was determined to be 60.9 ng/mL in DENSPM-stimulated HeLa cell extracts based on a 3.0 mg/mL extract load.

  • Sandwich ELISA sample test of MP00344-4

    Sandwich ELISA sample test of MP00344-4

    The mean SAT1 concentration was determined to be 62.4 ng/mL in U-251 cell extract based on a 1.4 mg/mL extract load.

  • Sandwich ELISA standard curve of MP00344-4

    Sandwich ELISA standard curve of MP00344-4

    Sandwich ELISA standard curve of MP00344-4, Human SAT1 Recombinant Matched Antibody Pair - PBS only. 83319-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1154. 83319-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SAT1 Recombinant monoclonal antibody (83319-1-RR)

    IHC staining of mouse brain using 83319-1-RR (same clone as 83319-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83319-1-RR (SAT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83319-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SAT1 Recombinant monoclonal antibody (83319-1-RR)

    IHC staining of mouse brain using 83319-1-RR (same clone as 83319-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83319-1-RR (SAT1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83319-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using SAT1 Recombinant monoclonal antibody (83319-1-RR)

    IF Staining of RAW 264.7 using 83319-1-RR (same clone as 83319-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using SAT1 antibody (83319-1-RR, Clone: 240157G2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83319-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of RAW 264.7 cells using SAT1 Recombinant monoclonal antibody (83319-1-RR)

    FC experiment of RAW 264.7 using 83319-1-RR (same clone as 83319-1-PBS)

    1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug SAT1 Recombinant antibody (83319-1-RR, Clone:240157G2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83319-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83319-1-PBS

    Affinity and Kinetic Characterization of 83319-1-PBS

    Biolayer interferometry (BLl) kinetic assay of 83319-1-PBS against Human SAT1 was performed. The affinity constant is 0.245 nM.

  • Affinity and Kinetic Characterization of 83319-1-RR

    Affinity and Kinetic Characterization of 83319-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83319-1-RR against Human SAT1 were performed. The affinity constant is 0.484 nM.

SAT1 Recombinant monoclonal antibody, PBS Only (Capture)

SAT1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA
Cat No. 83319-1-PBS
Clone No.240157G2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Synonyms

240157G2, EC:2.3.1.57, Polyamine N-acetyltransferase 1, SAT, Spermidine/spermine N(1)-acetyltransferase 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83319-1-PBS targets SAT1 as part of a matched antibody pair:

MP00344-4: 83319-1-PBS capture and 83319-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1154

Product name: Recombinant human SAT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-171 aa of BC008424

Sequence: MAKFVIRPATAADCSDILRLIKELAKYEYMEEQVILTEKDLLEDGFGEHPFYHCLVAEVPKEHWTPEGHSIVGFAMYYFTYDPWIGKLLYLEDFFVMSDYRGFGIGSEILKNLSQVAMRCRCSSMHFLVAEWNEPSINFYKRRGASDLSSEEGWRLFKIDKEYLLKMATEE

相同性解析による交差性が予測される生物種
Full Name spermidine/spermine N1-acetyltransferase 1
Calculated molecular weight 20 kDa
Observed molecular weight 15-25 kDa
GenBank accession numberBC008424
Gene Symbol SAT1
Gene ID (NCBI) 6303
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP21673
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Spermidine/spermine N1-acetyltransferase 1 (SAT1 or SSAT1) is the key regulatory enzyme in the catabolism of polyamines, catalyzing acetylation of spermidine or spermine to generate N1-acetyl spermidine or N1-acetyl spermine, and N1, N12-diacetylspermine. The cellular level of SAT1 is normally extremely low, but it is induced rapidly by a variety of stimuli, including polyamines, polyamine analogs, toxic chemicals, certain drugs, and growth factors. Downregulation of SAT1 has been reported in Epstein-Barr virus positive Burkitt's lymphoma cells.

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