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Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using Syntaxin 1B Recombinant antibody (83298-1-RR)

    WB analysis of Y79 using 83298-1-RR (same clone as 83298-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 83298-1-RR (STX1B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83298-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00312-1

    Sandwich ELISA standard curve of MP00312-1

    Sandwich ELISA standard curve of MP00312-1, Human STX1B Monoclonal Matched Antibody Pair - PBS only. 83298-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10275. 83298-4-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL.

  • Sample test of MP00312-1

    Sample test of MP00312-1

    The mean human STX1B concentration was determined to be 7.2 μg/mL in rat Brain tissue lysate based on a 3.0 mg/mL extract load. The mean human STX1B concentration was determined to be 18.1 μg/mL in mouse Brain tissue lysate based on a 2 mg/mL extract load. The mean human STX1B concentration was determined to be 7.0 ng/mL in rat kidney tissue lysate based on a 3 mg/mL extract load. The mean human STX1B concentration was determined to be 15.1 ng/mL in mouse kidney tissue lysate based on a 1.5 mg/mL extract load.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using Syntaxin 1B Recombinant antibody (83298-1-RR)

    IHC staining of mouse eye using 83298-1-RR (same clone as 83298-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 83298-1-RR (STX1B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83298-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using Syntaxin 1B Recombinant antibody (83298-1-RR)

    IHC staining of mouse eye using 83298-1-RR (same clone as 83298-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 83298-1-RR (STX1B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83298-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Syntaxin 1B Recombinant antibody (83298-1-RR)

    IHC staining of mouse brain using 83298-1-RR (same clone as 83298-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83298-1-RR (STX1B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83298-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Syntaxin 1B Recombinant antibody (83298-1-RR)

    IF Staining of mouse brain using 83298-1-RR (same clone as 83298-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STX1B antibody (83298-1-RR, Clone: 240156B9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83298-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Syntaxin 1B Recombinant antibody (83298-1-RR)

    IF Staining of mouse brain using 83298-1-RR (same clone as 83298-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STX1B antibody (83298-1-RR, Clone: 240156B9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83298-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using Syntaxin 1B Recombinant antibody (83298-1-RR)

    FC experiment of SH-SY5Y using 83298-1-RR (same clone as 83298-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Stx1b Recombinant Antibody (83298-1-RR, Clone:240156B9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83298-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00312-1

    Cytometric bead array standard curve of MP00312-1

    Cytometric bead array standard curve of MP00312-1, STX1B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83298-1-PBS. Detection antibody: 83298-4-PBS. Standard: Ag10275. Range: 1.25-40 ng/mL.

  • Affinity and Kinetic Characterization of 83298-1-PBS

    Affinity and Kinetic Characterization of 83298-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 83298-1-PBS against Human STX1B were performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of 83298-1-RR

    Affinity and Kinetic Characterization of 83298-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83298-1-RR against Human STX1B were performed. The affinity constant is below 1 pM.

Syntaxin 1B Recombinant monoclonal antibody, PBS Only (Capture)

Syntaxin 1B Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83298-1-PBS
Clone No.240156B9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Syntaxin 1B, STX1B, Syntaxin 1A, 240156B9, STX1B1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83298-1-PBS targets Syntaxin 1B as part of a matched antibody pair:

MP00312-1: 83298-1-PBS capture and 83298-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag10275

Product name: Recombinant human STX1B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-263 aa of BC062298

Sequence: MKDRTQELRSAKDSDDEEEVVHVDRDHFMDEFFEQVEEIRGCIEKLSEDVEQVKKQHSAILAAPNPDEKTKQELEDLTADIKKTANKVRSKLKAIEQSIEQEEGLNRSSADLRIRKTQHSTLSRKFVEVMTEYNATQSKYRDRCKDRIQRQLEITGRTTTNEELEDMLESGKLAIFTDDIKMDSQMTKQALNEIETRHNEIIKLETSIRELHDMFVDMAMLVESQGEMIDRIEYNVEHSVDYVERAVSDTKKAVKYQSKARRK

相同性解析による交差性が予測される生物種
Full Name syntaxin 1B
Calculated molecular weight 288 aa, 33 kDa
Observed molecular weight 33-35 kDa
GenBank accession numberBC062298
Gene Symbol STX1B
Gene ID (NCBI) 112755
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP61266
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and endocytic pathways (PMID: 11737951). Syntaxin 1A and 1B, two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641).

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