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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MLLT1 Recombinant antibody (83242-3-RR)

    WB analysis using 83242-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83242-3-RR (MLLT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MLLT1 Recombinant antibody (83242-3-RR)

    WB analysis of HeLa using 83242-3-RR

    WB result of MLLT1 antibody (83242-3-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MLLT1 transfected HeLa cells.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MLLT1 Recombinant antibody (83242-3-RR)

    IF Staining of HeLa using 83242-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MLLT1 antibody (83242-3-RR, Clone: 230519E7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using MLLT1 Recombinant antibody (83242-3-RR)

    FC experiment of HeLa using 83242-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MLLT1 Recombinant antibody (83242-3-RR, Clone:230519E7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83242-3-RR

    Affinity and Kinetic Characterization of 83242-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83242-3-RR against Human MLLT1 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

MLLT1 Recombinant monoclonal antibody

MLLT1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83242-3-RR
Clone No.230519E7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

230519E7, ENL, LTG19, MLLT 1, Protein ENL

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, Raji cells, K-562 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83242-3-RR targets MLLT1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34210

Product name: Recombinant human MLLT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 130-480 aa of BC148806

Sequence: EFRYKLLRAGGVMVMPEGADTVSRPSPDYPMLPTIPLSAFSDPKKTKPSHGSKDANKESSKTSKPHKVTKEHRERPRKDSESKSSSKELEREQAKSSKDTSRKLGEGRLPKEEKAPPPKAAFKEPKMALKETKLESTSPKGGPPPPPPPPPRASSKRPATADSPKPSAKKQKKSSSKGSRSAPGTSPRTSSSSSFSDKKPAKDKSSTRGEKVKAESEPREAKKALEVEESNSEDEASFKSESAQSSPSNSSSSSDSSSDSDFEPSQNHSQGPLRSMVEDLQSEESDEDDSSSGEEAAGKTNPGRDSRLSFSDSESDNSADSSLPSREPPPPQKPPPPNSKVSGRRSPESCS

相同性解析による交差性が予測される生物種
Full Name myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 1
Calculated molecular weight62 kDa
Observed molecular weight75 kDa
GenBank accession numberBC148806
Gene Symbol MLLT1
Gene ID (NCBI) 4298
RRIDAB_3670918
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ03111
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for MLLT1 antibody 83242-3-RRDownload protocol
IF protocol for MLLT1 antibody 83242-3-RRDownload protocol
WB protocol for MLLT1 antibody 83242-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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