Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, HEK-293 cells, mouse brain tissue, PC-3 cells, SH-SY5Y cells, HeLa cells, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83219-4-RR targets TMEM65 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag15509 Product name: Recombinant human TMEM65 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-178 aa of BC032396 Sequence: MEALNTAQGARDFIYSLHSTERSCLLKELHRFESIAIAQEKLEAPPPTPGQLRYVFIHNAIPFIGFGFLDNAIMIVAGTHIEMSIGIILGISTMAAAALGNLVSDLAGLGLAGYVEALASRLGLSIPDLTPKQVDMWQTRLSTHLGKAVGVTIGCILGMFPLIFFGGGEEDEKLETKS 相同性解析による交差性が予測される生物種 |
| Full Name | transmembrane protein 65 |
| Calculated molecular weight | 240 aa, 26 kDa |
| Observed molecular weight | 18 kDa |
| GenBank accession number | BC032396 |
| Gene Symbol | TMEM65 |
| Gene ID (NCBI) | 157378 |
| RRID | AB_3670904 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q6PI78 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TMEM65 (Transmembrane protein 65) is an inner-mitochondrial-membrane protein essential for calcium homeostasis and organelle integrity. By binding to and stabilizing the Na⁺/Ca²⁺ exchanger NCLX, it facilitates mitochondrial Ca²⁺ efflux, thereby regulating oxidative phosphorylation and limiting ROS production. In cardiomyocytes, TMEM65 localizes to intercalated discs where it interacts with connexin-43, ensuring normal electrical conduction and preventing arrhythmia. Loss-of-function mutations cause mitochondrial myopathy with neurodevelopmental delay, whereas its over-expression in gastric cancer activates the YWHAZ-PI3K-Akt-mTOR axis, promoting tumorigenesis. Thus, TMEM65 links mitochondrial quality control to cytoplasmic signaling, positioning it as a potential therapeutic target in metabolic and oncologic diseases.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TMEM65 antibody 83219-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |