Validation Data Gallery
Tested Applications
| Positive WB detected in | LNCaP cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83121-4-RR targets GCNT1 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag12264 Product name: Recombinant human GCNT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 41-428 aa of BC074885 Sequence: VSVRHLELAGENPSSDINCTKVLQGDVNEIQKVKLEILTVKFKKRPRWTPDDYINMTSDCSSFIKRRKYIVEPLSKEEAEFPIAYSIVVHHKIEMLDRLLRAIYMPQNFYCIHVDTKSEDSYLAAVMGIASCFSNVFVASRLESVVYASWSRVQADLNCMKDLYAMSANWKYLINLCGMDFPIKTNLEIVRKLKLLMGENNLETERMPSHKEERWKKRYEVVNGKLTNTGTVKMLPPLETPLFSGSAYFVVSREYVGYVLQNEKIQKLMEWAQDTYSPDEYLWATIQRIPEVPGSLPASHKYDLSDMQAVARFVKWQYFEGDVSKGAPYPPCDGVHVRSVCIFGAGDLNWMLRKHHLFANKFDVDVDLFAIQCLDEHLRHKALETLKH 相同性解析による交差性が予測される生物種 |
| Full Name | glucosaminyl (N-acetyl) transferase 1, core 2 (beta-1,6-N-acetylglucosaminyltransferase) |
| Calculated molecular weight | 428 aa, 50 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC074885 |
| Gene Symbol | GCNT1 |
| Gene ID (NCBI) | 2650 |
| RRID | AB_3670831 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q02742 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Glucosaminyl (N-acetyl) transferase 1 (GCNT1, also known as C2GNT1), is a glycosyltransferase enzyme that plays an essential role in the formation of core 2 branched O-glycans that is crucial to the final definition of O-glycan structure (PMID: 37813880). GCNT1 promotes the growth of prostate tumors and regulates oncogenic gene expression pathways that are important for disease progression (PMID: 24854630).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GCNT1 antibody 83121-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |