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GDNF Recombinant antibody

GDNF Recombinant Antibody for FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC, ELISA

Conjugate

Unconjugated

CloneNo.

230358A10

Cat no : 83087-1-RR

Synonyms

ATF, ATF1, ATF2, GDNF, HFB1 GDNF, hGDNF

Validation Data Gallery

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  • Flow cytometry (FC) experiment of U-251 cells using human GDNF Recombinant antibody (83087-1-RR)

    FC experiment of U-251 using 83087-1-RR

    (blue)1x10^6 U-251 cells were intracellularly stained with 0.25 ug Anti-Human GDNF (83087-1-RR, Clone:230358A10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using human GDNF Recombinant antibody (83087-1-RR)

    FC experiment of SH-SY5Y using 83087-1-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Anti-Human GDNF (83087-1-RR, Clone:230358A10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inSH-SY5Y cells, U-251 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83087-1-RR targets GDNF in FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GDNF fusion protein Ag24068
Full Name glial cell derived neurotrophic factor
Calculated Molecular Weight 211 aa, 24 kDa
Observed Molecular Weight 32-40 kDa
GenBank Accession NumberBC069369
Gene Symbol GDNF
Gene ID (NCBI) 2668
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glial-derived neurotrophic factor (GDNF) is a member of the transforming growth factor (TGF)-α family and exhibits potent neuroprotective activity. GDNF is a potent promoter of neuronal survival in the CNS and peripheral nervous system (PNS). It has a relatively high speciicity for dopaminergic neurons and thus become an efective treatment for Parkinson's diseases (PD), with clinical trials currently in progress. Protein dimers of GDNF have a reported molecular weight of 32-40 kDa(PMID: 17982417, PMID: 23251488, PMID: 11429269).

Protocols

Product Specific Protocols
FC protocol for GDNF antibody 83087-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols