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Validation Data Gallery

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  • Western Blot (WB) analysis of U266 cells using IFNAR2 Recombinant antibody (83068-5-RR)

    WB analysis of U266 using 83068-5-RR

    U266 cells were subjected to SDS PAGE followed by western blot with 83068-5-RR (IFNAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using IFNAR2 Recombinant antibody (83068-5-RR)

    WB analysis of HeLa using 83068-5-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 83068-5-RR (IFNAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using IFNAR2 Recombinant antibody (83068-5-RR)

    WB analysis of MCF-7 using 83068-5-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 83068-5-RR (IFNAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U266 cells using IFNAR2 Recombinant monoclonal antibody (83068-5-RR)

    IF Staining of U266 using 83068-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U266 cells using IFNAR2 antibody (83068-5-RR, Clone: 250313D3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

IFNAR2 Recombinant monoclonal antibody

IFNAR2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83068-5-RR
Clone No.250313D3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

IFN alpha REC, IFN alpha/beta receptor 2, IFN R, IFN R 2, IFNABR

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU266 cells, HeLa cells, MCF-7 cells
Positive IF/ICC detected inU266 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83068-5-RR targets IFNAR2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg2928

Product name: Recombinant Human IFNAR2 protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 27-243 aa of NM_207585.2

Sequence: ISYDSPDYTDESCTFKISLRNFRSILSWELKNHSIVPTHYTLLYTIMSKPEDLKVVKNCANTTRSFCDLTDEWRSTHEAYVTVLEGFSGNTTLFSCSHNFWLAIDMSFEPPEFEIVGFTNHINVMVKFPSIVEEELQFDLSLVIEEQSEGIVKKHKPEIKGNMSGNFTYIIDKLIPNTNYCVSVYLEHSDEQAVIKSPLKCTLLPPGQESESAESAK

相同性解析による交差性が予測される生物種
Full Name interferon (alpha, beta and omega) receptor 2
Calculated molecular weight58 kDa
Observed molecular weight90-100 kDa
GenBank accession numberNM_207585.2
Gene Symbol IFNAR2
Gene ID (NCBI) 3455
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP48551-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interferon alpha/beta receptor 2 (IFNAR2) is a type I membrane protein that associates with IFNAR1 to form the receptor for type I interferons. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2, and then increase transcription of the IFN-induced genes whose products exert antiviral, immunomodulatory, and antiproliferative effects.

Protocols

Product Specific Protocols
IF protocol for IFNAR2 antibody 83068-5-RRDownload protocol
WB protocol for IFNAR2 antibody 83068-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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