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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using Acetyl-Histone H2A (Lys9) Recombinant antibody (83041-1-RR)

    WB analysis of NIH/3T3 using 83041-1-RR

    Trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83041-1-RR (Acetyl-Histone H2A (Lys9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using Acetyl-Histone H2A (Lys9) Recombinant antibody (83041-1-RR)

    IHC staining of mouse lung using 83041-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83041-1-RR (Acetyl-Histone H2A (Lys9) antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using Acetyl-Histone H2A (Lys9) Recombinant antibody (83041-1-RR)

    IHC staining of mouse lung using 83041-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83041-1-RR (Acetyl-Histone H2A (Lys9) antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Acetyl-Histone H2A (Lys9) Recombinant antibody (83041-1-RR)

    IF Staining of HeLa using 83041-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2A (Lys9) antibody (83041-1-RR, Clone: 230349B1) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Acetyl-Histone H2A (Lys9) Recombinant antibody (83041-1-RR)

    IF Staining of HeLa using 83041-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2A (Lys9) antibody (83041-1-RR, Clone: 230349B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Acetyl-Histone H2A (Lys9) Recombinant antibody (83041-1-RR)

    IF Staining of HeLa using 83041-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2A (Lys9) antibody (83041-1-RR, Clone: 230349B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Dot Blot experiment of peptide using Acetyl-Histone H2A (Lys9) Recombinant antibody (83041-1-RR)

    Dot Blot experiment of peptide using 83041-1-RR

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H2A (Lys9) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.Column 1: H2AEK9Ac. Column 2: Unmodified H2AEK9. Column 3: H2AEK5Ac. Column 4: Unmodified H2AEK5. Column 5: H2AEK13Ac. Column 6: Unmodified H2AEK13. Column 7: H2AEK15Ac. Column 8: Unmodified H2AEK15. Column 9: Blank(PBS).

  • ChIP experiment of HeLa cells using Acetyl-Histone H2A (Lys9) Recombinant antibody (83041-1-RR)

    ChIP experiment of HeLa using 83041-1-RR

    Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H2A (Lys9) (83041-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

Acetyl-Histone H2A (Lys9) Recombinant monoclonal antibody

Acetyl-Histone H2A (Lys9) Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR
Cat No. 83041-1-RR
Clone No.230349B1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR

Synonyms

Ac Histone H2A, Ac Histone H2A (Lys9), Ac Histone H2A Lys9, Ac Histone H2A-Lys9, Acetyl Histone

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTrichostatin A treated NIH/3T3 cells
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive Dot Blot detected inpeptide
Positive ChIP-qPCR detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
DOT BLOTDOT BLOT : 1:10-1:100
CHIP-QPCRCHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83041-1-RR targets Acetyl-Histone H2A (Lys9) in WB, IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H2ae
Calculated molecular weight 14 kDa
Observed molecular weight14 kDa
GenBank accession numberBC093836
Gene Symbol HIST1H2AE
Gene ID (NCBI) 3012
RRIDAB_3670771
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP04908
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histone H2A is a core component of nucleosome. Histone variants contribute to chromatin complexity by creating specialized nucleosomes. Within nucleosomes, either one canonical H2A or both of them can be exchanged with a particular variant (heterotypic and homotypic nucleosomes, respectively), and such changes can have profound influences on nucleosome stability and biological outcome.

Protocols

Product Specific Protocols
IF protocol for Acetyl-Histone H2A (Lys9) antibody 83041-1-RRDownload protocol
IHC protocol for Acetyl-Histone H2A (Lys9) antibody 83041-1-RRDownload protocol
WB protocol for Acetyl-Histone H2A (Lys9) antibody 83041-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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