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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using RAB32 Recombinant antibody (82960-5-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82960-5-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82960-5-RR (RAB32 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using RAB32 Recombinant antibody (82960-5-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82960-5-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82960-5-RR (RAB32 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using RAB32 Recombinant antibody (82960-5-RR)

    IF Staining of U-251 using 82960-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RAB32 antibody (82960-5-RR, Clone: 230315B10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAB32 Recombinant antibody (82960-5-RR)

    IF Staining of HeLa using 82960-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAB32 antibody (82960-5-RR, Clone: 230315B10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using RAB32 Recombinant antibody (82960-5-RR)

    IF Staining of U-251 using 82960-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using RAB32 antibody (82960-5-RR, Clone: 230315B10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using RAB32 Recombinant antibody (82960-5-RR)

    FC experiment of HeLa using 82960-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAB32 Recombinant antibody (82960-5-RR, Clone:230315B10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82960-5-RR

    Affinity and Kinetic Characterization of 82960-5-RR

    Biolayer interferometry (BLl) kinetic assays of 82960-5-RR against Human RAB32 were performed. The affinity constant is 0.499 nM.

RAB32 Recombinant monoclonal antibody

RAB32 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 82960-5-RR
Clone No.230315B10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Ras-related protein Rab-32, Ras related protein Rab 32, 230315B10

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82960-5-RR targets RAB32 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1450

Product name: Recombinant human RAB32 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-225 aa of BC015061

Sequence: MAGGGAGDPGLGAAAAPAPETREHLFKVLVIGELGVGKTSIIKRYVHQLFSQHYRATIGVDFALKVLNWDSRTLVRLQLWDIAGQERFGNMTRVYYKEAVGAFVVFDISRSSTFEAVLKWKSDLDSKVHLPNGSPIPAVLLANKCDQNKDSSQSPSQVDQFCKEHGFAGWFETSAKDNINIEEAARFLVEKILVNHQSFPNEENDVDKIKLDQETLRAENKSQCC

相同性解析による交差性が予測される生物種
Full Name RAB32, member RAS oncogene family
Calculated molecular weight 25 kDa
GenBank accession numberBC015061
Gene Symbol RAB32
Gene ID (NCBI) 10981
RRIDAB_3670706
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ13637
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab32 belongs to the Ras-like small GTPase superfamily, which regulate membrane dynamics, including membrane fusion/fission, exocytosis, and cytoskeletal trafficking. Rab32 anchors the type II regulatory subunit of protein kinase A to the mitochondrion and aids in mitochondrial fission. TRab32 also appears to be involved in autophagy and melanosome secretion.

Protocols

Product Specific Protocols
IHC protocol for RAB32 antibody 82960-5-RRDownload protocol
IF protocol for RAB32 antibody 82960-5-RRDownload protocol
FC protocol for RAB32 antibody 82960-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols