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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using EMD Recombinant antibody (82888-2-RR)

    IHC staining of human thyroid cancer using 82888-2-RR

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 82888-2-RR (Emerin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using EMD Recombinant antibody (82888-2-RR)

    IHC staining of human thyroid cancer using 82888-2-RR

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 82888-2-RR (Emerin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Emerin Recombinant monoclonal antibody (82888-2-RR)

    IF Staining of HeLa using 82888-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Emerin antibody (82888-2-RR, Clone: 230089C4 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Emerin Recombinant antibody (82888-2-RR)

    IF Staining of HeLa using 82888-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Emerin antibody (82888-2-RR, Clone: 230089C4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using Emerin Recombinant monoclonal antibody (82888-2-RR)

    FC experiment of HeLa using 82888-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.13 ug Anti-Human Emerin (82888-2-RR, Clone:230089C4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.13 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 82888-2-RR

    Affinity and Kinetic Characterization of 82888-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82888-2-RR against Human Emerin were performed. The affinity constant is 1.71 nM.

Emerin Recombinant monoclonal antibody

Emerin Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 82888-2-RR
Clone No.230089C4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

EMD, 230089C4, EDMD, LEMD5, STA

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82888-2-RR targets Emerin in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0236

Product name: Recombinant human EMD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 66-252 aa of BC000738

Sequence: STRGDADMYDLPKKEDALLYQSKGYNDDYYEESYFTTRTYGEPESAGPSRAVRQSVTSFPDADAFHHQVHDDDLLSSSEEECKDRERPMYGRDSAYQSITHYRPVSASRSSLDLSYYPTSSSTSFMSSSSSSSSWLTRRAIRPENRAPGAGLGQDRQVPLWGQLLLFLVFVIVLFFIYHFMQAEEGN

相同性解析による交差性が予測される生物種
Full Name emerin
Calculated molecular weight 34 kDa
GenBank accession numberBC000738
Gene Symbol EMD
Gene ID (NCBI) 2010
RRIDAB_3670609
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP50402
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for Emerin antibody 82888-2-RRDownload protocol
IHC protocol for Emerin antibody 82888-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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