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Validation Data Gallery

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  • Sandwich ELISA sample test of MP02483-2

    Sandwich ELISA sample test of MP02483-2

    The mean CAMKK2 concentration was determined to be 24.41 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load, 8.92 ng/mL in PC-3 cell extract based on a 1.3 mg/mL extract load and 18.47 ng/mL in THP-1 cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02483-2

    Sandwich ELISA standard curve of MP02483-2

    Sandwich ELISA standard curve of MP02483-2, Human CAMKK2 Recombinant Matched Antibody Pair - PBS only. 82720-15-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2093. 82720-12-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

  • Cytometric bead array sample test of MP02483-1

    Cytometric bead array sample test of MP02483-1

    The mean CAMKK2 concentration was determined to be 1.6 ng/mL in PC-3 cell extract based on a 1.1 mg/mL extract load, 6.1 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load, 3.9 ng/mL in THP-1 cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP02483-1

    Cytometric bead array standard curve of MP02483-1

    Cytometric bead array standard curve of MP02483-1, CAMKK2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82720-12-PBS. Detection antibody: 82720-11-PBS. Standard: Ag2093. Range: 0.469-60 ng/mL.

CAMKK2 Recombinant monoclonal antibody, PBS Only (Capture/Detector)

CAMKK2 Uni-rAb® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 82720-12-PBS
Clone No.251379G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

Calcium/calmodulin-dependent protein kinase kinase 2, Calcium/calmodulin-dependent protein kinase kinase beta, CaM kinase kinase 2, CaM kinase kinase beta, CaM KK 2

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82720-12-PBS targets CAMKK2 as part of a matched antibody pair:

MP02483-1: 82720-12-PBS capture and 82720-11-PBS detection (validated in Cytometric bead array)

MP02483-2: 82720-15-PBS capture and 82720-12-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2093

Product name: Recombinant human CAMKK2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-353 aa of BC026060

Sequence: MSSCVSSQPSSNRAAPQDELGGRGSSSSESQKPCEALRGLSSLSIHLGMESFIVVTECEPGCAVDLGLARDRPLEADGQEVPLDSSGSQARPHLSGRKLSLQERSQGGLAAGGSLDMNGRCICPSLPYSPVSSPQSSPRLPRRPTVESHHVSITGMQDCVQLNQYTLKDEIGKGSYGVVKLAYNENDNTYYAMKVLSKKKLIRQAGFPRRPPPRGTRPAPGGCIQPRGPIEQVYQEIAILKKLDHPNVVKLVEVLDDPNEDHLYMVFELVNQGPVMEVPTLKPLSEDQARFYFQDLIKGIEYLHYQKIIHRDIKPSNLLVGEDGHIKIADFGVSNEFKGSDALLSNTVGTPAF

相同性解析による交差性が予測される生物種
Full Name calcium/calmodulin-dependent protein kinase kinase 2, beta
Calculated molecular weight 588 aa, 60 kDa
GenBank accession numberBC026060
Gene Symbol CAMKK2
Gene ID (NCBI) 10645
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ96RR4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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