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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GCLM Recombinant antibody (82623-1-RR)

    WB analysis using 82623-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82623-1-RR (GCLM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GCLM Recombinant antibody (82623-1-RR)

    WB analysis of HeLa using 82623-1-RR

    WB result of GCLM antibody (82623-1-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GCLM transfected HeLa cells.

  • Western Blot (WB) analysis of RAW 264.7 cells using GCLM Recombinant antibody (82623-1-RR)

    WB analysis of RAW 264.7 using 82623-1-RR

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 82623-1-RR (GCLM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GCLM Recombinant antibody (82623-1-RR)

    IHC staining of mouse brain using 82623-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82623-1-RR (GCLM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GCLM Recombinant antibody (82623-1-RR)

    IHC staining of mouse brain using 82623-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82623-1-RR (GCLM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovarian cancer using GCLM Recombinant antibody (82623-1-RR)

    IHC staining of human ovarian cancer using 82623-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82623-1-RR (GCLM antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovarian cancer using GCLM Recombinant antibody (82623-1-RR)

    IHC staining of human ovarian cancer using 82623-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82623-1-RR (GCLM antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using GCLM Recombinant antibody (82623-1-RR)

    IHC staining of human skin cancer using 82623-1-RR

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82623-1-RR (GCLM antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using GCLM Recombinant antibody (82623-1-RR)

    IHC staining of human skin cancer using 82623-1-RR

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82623-1-RR (GCLM antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82623-1-RR

    Affinity and Kinetic Characterization of 82623-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82623-1-RR against Human GCLM were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

GCLM Recombinant monoclonal antibody

GCLM Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 82623-1-RR
Clone No.4K16

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Glutamate--cysteine ligase regulatory subunit, Glutamate--cysteine ligase modifier subunit, GLCLR, Gamma-glutamylcysteine synthetase regulatory subunit, Gamma-ECS regulatory subunit

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, RAW 264.7 cells, HEK-293 cells, A431 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IHC detected inmouse brain tissue, human ovarian cancer, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82623-1-RR targets GCLM in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag5497

Product name: Recombinant human GCLM protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-274 aa of BC041809

Sequence: MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS

相同性解析による交差性が予測される生物種
Full Name glutamate-cysteine ligase, modifier subunit
Calculated molecular weight 31 kDa
Observed molecular weight31 kDa
GenBank accession numberBC041809
Gene Symbol GCLM
Gene ID (NCBI) 2730
RRIDAB_3086493
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP48507
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GCLM(Glutamate--cysteine ligase regulatory subunit) is also named as GLCLR and belongs to the aldo/keto reductase family. It catalyzes the first step of GSH synthesis and is involved in gamma-glutamyl cycle. GCLM is implicated in some forms of hemolytic anemia.

Protocols

Product Specific Protocols
WB protocol for GCLM antibody 82623-1-RRDownload protocol
IHC protocol for GCLM antibody 82623-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols