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Validation Data Gallery

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  • Sandwich ELISA standard curve of MP50033-1

    Sandwich ELISA standard curve of MP50033-1

    Sandwich ELISA standard curve of MP50033-1, MCSF Monoclonal Matched Antibody Pair - PBS Only. 69033-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard HZ-1192. 68662-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Sample test of MP50033-1

    Sample test of MP50033-1

    Serum of sixteen individual healthy human donors was measured. The M-CSF concentration of detected samples was determined to be 712.6 pg/mL with a range of 223.9-2,041.6 pg/mL.

  • Sample test of MP50033-1

    Sample test of MP50033-1

    Urine of six individual healthy human donors was measured. The M-CSF concentration of detected samples was determined to be 1,272.6 pg/mL with a range of 216.3-2,082.1 pg/mL.

  • Sample test of MP50033-1

    Sample test of MP50033-1

    Saliva of three individual healthy human donors was measured. The M-CSF concentration of detected samples was determined to be 185.5 pg/mL with a range of 21.3-473.0 pg/mL.

  • Neutralization experiment of NeutraKine®M-CSF using NeutraKine®M-CSF Mouse McAb (69033-1-Ig)

    Neutralization experiment of NeutraKine®M-CSF using 69033-1-Ig (same clone as 69033-1-PBS)

    Recombinant human M-CSF(Cat.NO. HZ-1192) stimulates the proliferation of OCI-AML5 cells in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human M-CSF (5 ng/mL) is neutralized by mouse anti-human M-CSF monoclonal antibody 69033-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 0.1-0.4 μg/mL. The control mouse anti-human M-CSF monoclonal antibody 69533-1-Ig could recognize human M-CSF, but could not neutralize human M-CSF at serial does at the range of 0.976563-16000ng/mL(green curve, refer to top X-right Y). This data was developed using the same antibody clone with 69033-1-PBS in a different storage buffer formulation.

  • Neutralization experiment of NeutraKine®M-CSF using NeutraKine®M-CSF Mouse McAb (69033-1-Ig)

    Neutralization experiment of NeutraKine®M-CSF using 69033-1-Ig (same clone as 69033-1-PBS)

    Recombinant human M-CSF (Cat.NO. HZ-1192) stimulates proliferation of OCI-AML5 cells in a dose dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human M-CSF (5ng/mL HZ-1192) is neutralized by mouse anti-human M-CSF monoclonal antibody 69033-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 0.1-0.4 μg/mL. This data was developed using the same antibody clone with 69033-1-PBS in a different storage buffer formulation.

  • ELISA experiment of Recombinant protein using NeutraKine®M-CSF Mouse McAb (69033-1-Ig)

    ELISA experiment of Recombinant protein using 69033-1-Ig (same clone as 69033-1-PBS)

    Indirect ELISA was carried out by coating recombinant Human M-CSF (Cat.NO. HZ-1192) at 70 ng/well followed by blocking and adding serial diluted M-CSF antibody 69033-1-Ig and 69533-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 69033-1-PBS in a different storage buffer formulation.

NeutraKine®M-CSF Monoclonal antibody, PBS Only (Capture)

NeutraKine®M-CSF Monoclonal Antibody for Neutralization, ELISA, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 69033-1-PBS
Clone No.4C6C5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Neutralization, ELISA, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

M-CSF, 4C6C5, CSF 1, CSF1, CSF-1

Formulation:  PBS Only
Sterile PBS
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Product Information

69033-1-PBS targets NeutraKine®M-CSF as part of a matched antibody pair:

MP50033-1: 69033-1-PBS capture and 68662-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Affinity
KD=8.48 x 10-9M
KOff=4.75 x 10-4M
KOn=5.60 x 104M
Immunogen

Recombinant Protein

相同性解析による交差性が予測される生物種
Full Name colony stimulating factor 1 (macrophage)
Gene Symbol M-CSF
Gene ID (NCBI) 1435
Conjugate Unconjugated
Form
Purification MethodProtein G purification
UNIPROT IDP09603
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

M-CSF plays an essential role in the regulation of survival, proliferation, and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates the formation of membrane ruffles, cell adhesion, and cell migration, and plays a role in lipoprotein clearance.

This antibody is used to neutralize the bioactivity of M-CSF.